Effect on cell cultures

It is therefore important when attempting to grow mycoplasma to use media enriched in, for instance, yeast extract which supplies all essential nutrients. In addition, some mycoplasma grow better under anaerobic conditions. 

Kenny (1973) recommends a Soy peptone-yeast dialysate medium (Appendix 4) for culture of mycoplasma. Inclusion of arginine (16 mM) and 0.4 mg% phenol red indicates the presence of arginine deaminase by formation of alkali (purple coloration). Alternatively, incubation with tritiated thymidine and analysis of the culture medium for tritiated thymine can be used to detect thymidine phosphorylase (House and Waddell, 1967). 

A simpler medium based on the formula of Hayflick (1965b) is in use in our laboratory. 

70 parts 3% brain-heart infusion (Difco Labs) 

10 parts autoclaved yeast dialysate (or extract) supplemented with 0.5% glucose, arginine HC1 and phenol red together with penicillin G (10,000 U/ml) and thallium acetate (0.05%). 

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A method of assessing the toxicity of implants has been proposed based on the effects on cell ultrastructure in organ cultures, on cell surface characteristics, and cell population doubling times. The effects have been correlated with hemorrhage, fibrosis, and necrosis, respectively (103). Poly-e-caprolactone was stated to give minimal tissue reaction and could not be scored in these tests. 

Biological Effects in Cell Cultures of Fullerene C60 Dependence on Aggregation State... 

Table 7.3 Photodynamic action of fullerene on the surfaces (FoS) (lOpg/cm2 of Cf/)) on cell culture MA-104 under effect of some antioxidants and Na-azide...

Eropkin MYu, Eropkina EM, Kiselev OI (2007) Studying the effect of antioxidants and/or anti-hypoxants on cell cultures under conditions of cytotoxic action of rimantadine. Exp Clin Pharmacol. 70 56-61 (Russian). 

Lubart R, Wollman Y, Friedmann H, Rochkind S, Laulicht I (1992) Effects of visible and near-infrared lasers on cell cultures. J Photochem Photobiol B 12 305-310... 

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