Two-color systems

Usually a 1 to 2 unit change in pH is required to convert the dye from one color to the other. In a two-color system, one color is discernible only if the species giving rise to that color is in ten-fold excess over the alternative species. Therefore,... 

Tunel (Terminal deoxynucleotide transferase dUTP nick end labeUng) assay In a modified version of the Tunel assay, apoptotic ceUs can be measured in a two-color system using flow cytometry and microscopy. In this assay, DNA breaks are labeled by deoxynucleotidyl transferase using bromodeoxyuridine triphosphate (BrdUTP) and visualized by anti-BrdU antibody. Propidium iodide is used to counterstain the total ceUular DNA. 

Limitation of the measurement volume size by additional optical systems (i.e. gate photodetector (Chigier et al. 1979) or two-color systems with two measurement volumes of different diameter (Yeoman et al. 1982)). 

Continuous mode ink-jet systems are widely used in industrial market, mainly for product labeling of food and medicines. They have high throughput capabiUties, especially array continuous mode systems, and are best suited for high duty cycle applications. Few continuous ink-jet systems are multicolor (multifluid) but two-color systems are in use. 

Two-Color Systems By measuring the irradiance at two different wavelengths and determining their ratio, we can determine the temperature of the source, independent of its emissivity. Temperature determined in this way is referred to as the color temperature. 

Blt-M ppedImages. A bit map is a grid pattern composed of tiny cells or picture elements called pixels. Each pixel has two attributes a location and a value or set of values. Location is defined as the address of the cell in a Cartesian, ie, x andjy coordinate, system. Value is defined as the color of the pixel in a specified color system. Geometric quaUties of images are a function of the location attribute, ie, the finer the grid pattern, the more precisely can the geometric quaUties be controlled. Color quaUties are a function of the value attribute, ie, the more bytes of computer memory assigned to describe each pixel, the more precisely can the color quaUties be controlled. 

In color technology and measurement, both types of approaches are used. Color printing, for example, generally employs three colors (usually plus black), and the ever useful CIE system was founded on experiments in which colors were matched by mixtures of three primary colors, often blue, green, and red. Yet transmitted television signals are based on the opponent system, with one intensity and two color-balance signals, as are the modern representations of color, such as the CIELAB and related color spaces based on red-green and yeUow-blue opponent axes. 

D Shalon, SJ Smith, PO Brown. A DNA microairay system for analyzing complex DNA samples using two-color fluorescent probe hybridization. Genome Res 6 639-645, 1996. MB Eisen, PT Spellman, PO Brown, D Botstem. Cluster analysis and display of genomewide expression patterns. Proc Natl Acad Sci USA 95 14863-14868, 1998. 

When Munsell devised his color space, he did so on the basis of minimum observable color perception steps. But the problem with the Munsell System was one of reproducibility, which the CIE Standard Observer cured. In formulating a color match, one wants to be able to predict the correct concentration of colorants required, whose scattering and absorption properties are known, i.e.- the lightness, so as to match the sample submitted, starting with their spectrophotometric curves. In practice, this is not so simple, since two colors must have identical spectrophotometric curves to be exactly equal. It turns out that the human eye will identify the two colors to be equal if their spectrophotometric reflectances are reasonably close. Two colors may appear to be equal under Daylight illumination, but quite different under incandescent lamp illumination. These colors are known zus "metamers" and the phenomenon "metamerism". 

As the plastic liners are removed from the probe, they are capped on both ends, the appropriate labels affixed, and promptly placed in a freezer (an in-field sectioning technique used for further partitioning of the 0-15-cm core is described later in this section). By convention, red plastic caps are placed on top of the core (i.e., the end that was closest to the soil surface) and black caps are placed on the bottom. Use of the two-color capping system is important when the cores are sectioned at a later time. This approach is referred to as zero-contamination sampling and is the industry standard in field soil dissipation. 

Petersen, M., L. Wiking, and H. Stapelfeldt. 1999. Light sensitivity of two colorants for Cheddar cheese. Quantum yields for photodegradation in an aqueous model system in relation to light stability of cheese in illuminated display. J. Dairy Res. 66 599-607. 

Synthesis of novel polyelectrochromic materials with the new structural motif utilizing stabilized carbocations has been demonstrated by several examples. The Wurster type violene-cyanine hybrid systems (22z+ and 232+) exhibit the presumed two color changes. The new cyanine-cyanine hybrid system (24 ) with a cyanine unit at the one terminus exhibits three color changes. 

In a first step we will analyze the dominant condensation pattern in the scalar color antitriplet channel for a two-flavor system, i.e., we will consider the limit Ms —> 00. Looking at the transformation properties of the condensate S22 we can classify the symmetries of the 2SC phase. It is invariant under a global //(l)-symmetry generated by... 

In colorimetric terms, color differences are generally characterized by the distance between two colors in one of the color notation systems, most commonly the CIELAB system. The difference determined in this way represents the total color difference. Since a color is characterized by three quantities, a color difference can also be expressed in terms of three difference components (that is, differences in the color coordinates) and thus described in greater detail. Visually equal color differences between almost equal colors may, upon colorimetric measurement, be considered distinct if the colors of one pair differ strongly from those of the other pair. The formulas for color differences are further elaborated in order to remedy this defect. 

Fig. 23. This photograph shows a two-phase system consisting of (top phase) toluene and (bottom phase) perfluoro-2-butyltetrahydrofuran (Fluoroinert FC-75).The dark color (brown) in the bottom phase indicates that the dendrimer-encapsulated Pd nanoparticles, complexed with poly (hexafluoropropylene oxide-co-difluoromethylene oxide) monocarboxylic acid, are selectively extracted into the fluorous phase. No detectable color was observed in the organic phase. Reprinted with permission from Ref. 103 Copyright 2000 American Chemical Society...

There are times when two color assays may be required. This can be accomplished by altering the enzyme system used for one of the assays or the chromogen used for one of the assays if the enzymes are not altered. Perhaps the easiest method is to alter the color product of the DAB chromogen for one of the assays. This can be done be adding a heavy metal compound such as cobalt or nickel to the dilute solution (9). The resultant precipitate will be blue to black, not brown. It is helpful in this circumstance to use a Methyl Green counterstain, which will enhance the black precipitate and avoid the confusion of color seen with some other counter stains. 

A) Diazo-coupling in a two-phase system using BCR diazotized sulfanilic acid the azo color, obtained in the aqueous phase, is determined photometrically... 

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