Tumor necrosis factor, recombinant

Enbrel. See Tumor necrosis factor, recombinant Encloiniphene. 781... 

In E. Coli bacterial lysates, the proteome (i.e., the full array of proteins produced) was analyzed by isoelectric focusing and mass spectrometry.97 A comparison of capillary electrophoretic separation and slab gel separation of a recombinant monoclonal antibody demonstrated that the precision, robustness, speed, and ease-of-use of CE were superior.98 Seventy-five proteins from the yeast ribosome were analyzed and identified by capillary electrophoresis coupled with MS/MS tandem mass spectrometry.99 Heavy-chain C-terminal variants of the anti-tumor necrosis factor antibody DE7 have been separated on capillary isoelectric focusing.100 Isoforms differing by about 0.1 pi units represented antibodies with 0,1 or 2 C-terminal lysines. 

Etanercept is a recombinant form of human tumor necrosis factor receptor.24 Etanercept provides a therapeutic effect by binding to soluble TNF and preventing its binding with TNF receptors. It is administered subcutaneously once or twice weekly noticeable symptomatic improvement is seen in 1 to... 

C25. Cross, A. S., Sadoff, J. C., Kelly, N., Bemton, E., and Gemski, P., Pretreatment with recombinant murine tumor necrosis factor-alpha/cachectin and murine interleukin 1 -alpha protects mice from lethal bacterial infection. J. Exp. Med. 169,2021-2027 (1989). 

Recombinant tumor necrosis factor and interleukin 1 enhance slow-wave sleep. Am. J. Physiol. 253, R142-9. 

Corazza, N., Eichenberger, S., Eugster, H.P. and Mueller, C. (1999) Nonlymphocyte-derived tumor necrosis factor is required for induction of colitis in recombination activating gene (RAG)2( / ) mice upon transfer of CD4(+)CD45RB(hl) T cells. Journal of Experimental Medicine 190,1479-1492. 

Mammalian cell suspension cultures are the preferred choice for large-scale recombinant protein production in stirred-tank bioreactors. The most widely used systems are Chinese hamster ovary (CHO) cells and the murine myeloma fines NSO and SP2/0. In half of the biological license approvals from 1996-2000, CHO cells were used for the production of monoclonal antibodies and other recombinant glycosylated proteins, including tPA (tissue plasminogen activator) and an IgGl fusion with the tumor necrosis factor (TNF) receptor, the latter marketed as Enbrel [7]. 

Talmadge, J.E. et al., Immunomodulatory and immunotherapeutic properties of recombinant gamma- interferon and recombinant tumor necrosis factor in mice, Cancer Res, 47, 2563, 1987. 

Gamble, J.R. et al., Stimulation of the adherence of neutrophils to umbilical vein endothelium by human recombinant tumor necrosis factor, Proc Natl Acad Sci U.S.A, 82, 8667, 1985. 

Wing, E J. et al., Recombinant human granulocyte/macrophage colony-stimulating factor enhances monocyte cytotoxicity and secretion of tumor necrosis factor alpha and interferon in cancer patients, Blood, 73, 643, 1989. 

Examples of the early application of recombinant DNA technology in medicine are the development of recombinant human growth hormone human insulin human interferons, thought to have anticancer activity in addition to antiviral activity interleukins (regulatory proteins from lymphocytes that are believed to be important in the treatment of immunodeficiency diseases and cancer) tumor necrosis factor epidermal and bone marrow progenitor cell growth factors and the production of vaccines (Table 12.1). 

Recombinant human Tumor Necrosis Factor Purification, comparison with conventional columns Ion Exchange disks, Hydro-phobic Interaction disks [72]... 

Savva M, Duda E, Huang L. A genetically modified recombinant tumor necrosis factor-alpha conjugated to the distal terminals of liposomal surface grafted polyethyleneglycol chains. Int J Pharm 1999 184 45. 

Etanercept is a recombinant human soluble tumor necrosis factor-alpha (TNFo ) receptor fusion protein that binds to TNFo and decreases its role in disorders involving excess inflammation. It is approved for subcutaneous use in the treatment of patients with moderate to severe active rheumatoid arthritis, juvenile rheumatoid arthritis, psoriatic arthritis, ankylosing arthritis and plaque psoriasis. To the adverse reactions mentioned for infliximab, rare reports of congestive heart failure should be added. 

Etanercept-Recombination Antirheumatic, tumor necrosis factor receptor... 

Because plasma protein and tissue binding influences the amount of drug in each body compartment, the volume of distribution of a drug may be dose dependent (nonhnear). A limited number of binding sites may result in capacity-limited binding in plasma or tissues. Transport from the blood may also be capacity limited. Examples of dose-dependent volume changes show that the volume of distribution of recombinant human tumor necrosis factor (TNF) alpha decreases sharply with a fourfold increase in dose and that the volume of distribution of recombinant human DNase,... 

DeLa Cadena, R.A., A. Majluf-Cruz, A. Stadnicki, M. Tropea, D. Reda, IM. Agosti, R.W. Colman, and A.F. Suffredini, Recombinant tumor necrosis factor receptor p75 fusion protein (TNFR Fc) alters endotoxin-induced activation of the kinin, fibrinolytic, and coagulation systems in normal humans. Thromb Haemost, 1998. 80(1) 114-8. 

Murray, K.M., and S.L. Dahl, Recombinant human tumor necrosis factor receptor (p75) Fc fusion protein (TNFR Fc) in rheumatoid arthritis. Ann Pharmacother, 1997. 31(11) 1335-8. 

Eason, ID., S.L. Wee,T. Kawai, H.Z. Hong, I Powelson, M. Widmer, and A.B. Cosimi, Recombinant human dimeric tumor necrosis factor receptor (TNFR Fc) as an immunosuppressive agent in renal allograft recipients. Transplant Proc, 1995. 27(1) 554. 

Cooke, S.P., R.B. Pedley, R. Boden, R.H. Begent, and K.A. Chester, In vivo tumor delivery of a recombinant single-chain Fvr.tumor necrosis factor a fusion protein. Bioconjug Chem, 2002.13(1) 7-15. 

Cheever, A.W., Poindexter, R.W. and Wynn, T.A. (1 999) Egg laying is delayed but worm fecundity is normal in SCID mice infected with Schistosoma japonicum and 5. mansoni with or without recombinant tumor necrosis factor alpha treatment. Infection and Immunity 67, 2201-2208. 

Moreland, L. W., Margolies, G., Heck, L. W., Jr. et al. (1996). Recombinant soluble tumor necrosis factor receptor (p80) fusion protein Toxicity and dose finding trial in refractory rheumatoid arthritis. J Rheumatol 23, 1849-1855. 

Weiss, J., Elsbach, P., Shu, C., Castillo, J., Grinna, L., Horwitz, A., Theofan, G. Human bactericidal/permeability-increasing protein and a recombinant NH2-terminal fragment cause killing of serum-resistant gram-negative bacteria in whole blood and inhibit tumor necrosis factor release induced by the bacteria. J Clin Invest 90 (1992) 1122-1130. 

Zeltser R, Valle L,Tanck C, Holyst MM, Ritchlin C, Gaspari AA. Clinical, histological, and immunophenotypic characteristics of injection site reactions associated with etanercept a recombinant tumor necrosis factor alpha receptor Fc fusion protein. Arch Dermatol 2001 137(7) 893-9. 

Hori K, Ehrke MJ, Mace K, Maccubbin D, Doyle MJ, Otsuka Y, Mihich E. Effect of recombinant human tumor necrosis factor on the induction of murine macrophage tumoricidal activity. Cancer Res 1987 47 2793-8. 

HGL920 CAS 94948-59-1 HR 3 HUMAN RECOMBINANT TUMOR NECROSIS FACTOR-a... 

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