Transcription species-specific

Bank PA, Yao EF, Phelps CL, Harper PA, Denison MS. 1992. Species-specific binding of transformed Ah receptor to a dioxin responsive transcriptional enhancer. Eur. J. Pharmacol. 258 85—94... 

Liu D, Wang C, Swiatlo EJ, Lawrence ML (2005) PCR amplification of a species-specific putative transcriptional regulator gene reveals the identity of Enterococcus faecalis. Microbiol Res 156 944-948... 

Rudloff U., Eberhard D., Tora L., Stunnenberg H., Grummt I. (1994) TBP-associated factors interact with DNA and govern species specificity of RNA polymerase I transcription. EMBO J 13 2611. 

Several publications describe assays for genomic animal DNA. Analysis of short interspersed nuclear elements (SINEs) allows specific detection of DNA from many species, including cattle, horse, pig, deer, and dog. SINEs are repeated, unblocked, and dispersed throughout the genome sequences. They represent retroposons (included in the genome transcripts of intracellular RNA) and constitute more than 20% of the genome of humans and other mammals. Unique sequences could be identified for every species and used for the development of a species-specific PCR assay (Walker et al., 2003). For this application the laboratory has to prepare its own control samples with the DNA extracted from reference samples. 

Seyboldt C, John A, Mueffling TV, Nowak B, Wenzel S (2003). Reverse transcription polymerase chain reaction assay for species-specific detection of bovine central nervous system tissue in meat and meat products. J. Food Prot., 66 644—651. 

Interspecific cell hybrids between human and murine cells do not express the rDNA genes of both genes. Interspecific crosses in plants and between Xenopus species have also shown a gene-specific dominance effect. This phenomenon is known as nucleolar dominance and, depending on the chromosomal organization of the hybrid, the rDNA of either one of the species is expressed (Reeder, 1985). These findings indicated that there are some aspects of rDNA transcription that are species specific. The incompatibility between different species has then been... 

An essential factor required for accurate RNA Pol I transcription is the selectivity factor SL1 (SL1 Clos et al., 1986a Learned et al., 1985). As mentioned earlier, SL1 is also a species-specific factor that directs promoter-specific transcription in the presence of its cognate template. SL1 does not bind specifically to the rRNA promoter however, in the presence of UBF, it forms a strong cooperative DNA-binding complex at the ribosomal DNA promoter that is critical for initiation of transcription (Bell et al., 1988, 1989 Learned et al., 1986). Mutations in the promoter sequences that affect either the binding of UBF to the DNA template or the interaction of UBF with SL1 result in drastic reduction of transcription activity (Bell et al., 1988). These findings indicate that the interactions between UBF and its DNA recognition sequence, and between UBF and SL1, play a major role in RNA Pol I transcription. For many years the molecular identification of SL1 has proven a difficult task. The... 

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