Trametes hirsuta

Couto SR, Sanroman MA, Hofer D, Giibitz GM (2004) Stainless steel sponge a novel carrier for the immobilization of the white-rot fungus Trametes hirsuta for decolorization of textile dyes. Bioresour Technol 95 67-72... 

Puri SG, Nazir A, Ghawla R, Arora R, Riyaz-ul-Hasan S, Amna T, Ahmed B, Verma V, Singh S, Sagar R, Sharma A, Kumar R, Sharma RK, Ghulam NQ, The endophytic fungus Trametes hirsuta as a novel alternative source of podophyllo-toxin and related aryl tetralin Yign2isi, J Biotechnol 122 494-510, 2006. 

Puri SC The endophytic fungus Trametes hirsuta zs z novel ahemzxive source of podophyllotoxin and related aryl tetrahn li zirs, J Biotechnol 122 494—510, 2006. 

Trametes hirsuta Other as above 399 55 Negligible ND ND ND ND 56 Field Lamar et al. (1993)... 

Chicken Schizo- phyllum commune Stereum sanguino- lenthum Trametes hirsuta Asper- gillus niger... 

Schizo- phyllum commune xylanase Stereum sanguino- lentum xylanase Trametes hirsuta xylanase Aspergillus niger xylanase Average Xylanase... 

Oxidation of two out of 13 tryptophan residues in a cellulase from Penicillium notatum resulted in a complete loss of enzymic activity (59). There was an interaction between cellobiose and tryptophan residues in the enzyme. Participation of histidine residues is also suspected in the catalytic mechanism since diazonium-l-H-tetrazole inactivated the enzyme. A xylanase from Trametes hirsuta was inactivated by N-bromosuc-cinimide and partially inactivated by N-acetylimidazole (60), indicating the possible involvement of tryptophan and tyrosine residues in the active site. As with many chemical modification experiments, it is not possible to state definitively that certain residues are involved in the active site since inactivation might be caused by conformational changes in the enzyme molecule produced by the change in properties of residues distant from the active site. However, from a summary of the available evidence it appears that, for many / -(l- 4) glycoside hydrolases, acidic and aromatic amino acid residues are involved in the catalytic site, probably at the active and binding sites, respectively. 

Kim S, Lopez C, Guebitz G et al (2008) Biological coloration of flax fabrics with flavonoids using laccase from Trametes hirsuta. Eng Life Sci 8 324-330... 

Puri SC, et al. The endoph)4ic fungus Trametes hirsuta as a novel alternative source of podophyllotoxin and related aryl tetrahn lignans. J. Biotechnol. 2006 122 494-510. 

Puri, S. C., Nazir, A., Chawla, R., Arora, R., Riyaz-ul-Hasan, S., Amna, T, Ahmed, B., Verma, V, Singh, S., Sagar, R., Sharma, A., Kumar, R., Sharma, R. K., Qazi, G. N. The endophytic fungus Trametes hirsuta as a novel alternative source of podophyllotoxin and related tetralin hgnans. J. Biotech. 2006,122, 494—510. 

K Messner, H Stachelberger. Transmission electron microscope observations of white rot caused by Trametes hirsuta with respect to osmiophilic particles. Trans Br Mycol Soc 83 209-216. 1984. 

Trametes hirsuta laccase Graphite Electrode for direct electrocatalytic reduction of 02 to H20 [82]... 

Trametes hirsuta laccase MWCNT modified GCE Biofuel cells [107]... 

Some properties of purified endo-(l- -4)- 3-D-xylanase from the ligniperdous fungus Trametes hirsuta have been investigated. The enzyme was stable between pH 4.0 and 8.0 with optimum activity at pH 5.0-5.5. The temperature optimum was 50 C and the enzyme was stable for up to 30 min at 45 C however, it was denatured at higher temperatures. The for 4-0-methyl-D-glucurono-D-xylan was 6.36 X10" equivalents of D-xylose per litre, the activation energy was 28 kj moF The enzyme (mol. wt. 2.2-2.4X 10 by gel chromatography) was activated by Ca " and inhibited by Ag and Hg. On the basis of the effects of 2-hydroxy-5-nitrobenzyl bromide, iV-bromosuccinimide, and A-acetylimidazole it was assumed that L-tryptophan and possibly L-tyrosine residues influence the enzyme catalysts. 

Haghighi, B., A. Rahmati-Panah, S. Shleev, and L. Gorton, 2007. Carbon ceramic electrodes modified with laccase from Trametes hirsuta Fabrication, characterization and their use for phenolic compounds. Electroanalysis 19 907-17. 

The approaches that are commonly used to improve the in vitro production of PTOX in plant organ cultures include optimization of cultivation, precursor feeding, and elicitation [96]. The highest amount of PTOX (2.26% DW) was achieved in the coculture of L. album suspension cells with the live fungus Sebacina ver-mifera [97]. In addition, several endophytic fungi cultures have the capacity to produce PTOX, such as Fusarium solani from P hexandrum [98], Phialocephala fortinii from P. peltatum [99], Trametes hirsuta from P. hexandrum [100], and... 

A xylanase has been isolated from a wood-rot fungus Trametes hirsuta, and the subunits of a xylanase from Trichoderma viride have been fractionated by preparative electrophoresis on polyacrylamide gel. ... 

Ackermann, Y., Guschin, D.A., Eckhard, K., Shleev, S., Schuhmann, W. Design of a bioelectrocatalytic electrode interface for oxygen reduction in biofuel cells based on a specifically adapted Os-complex containing redox polymer with entrapped Trametes hirsuta laccase. Electrochem. Commun. 2010,12, 640-643. 

Shleev S, Christenson A, Serezhenkov V, Burbaev D, Yaropolov A, Gorton L, Ruzgas T. Electrochemical redox transformations of T1 and T2 copper sites in native Trametes hirsuta laccase at a gold electrode. Biochem J 2005 385 745-754. 

Shleev S, Pita M, Yaropolov AI, Ruzgas T, Gorton L. Direct heterogeneous electron transfer reactions of Trametes hirsuta laccase at bare and thiol-modified gold electrodes. 

Wang X, Latonen R-M, Sjoberg-Eerola P, Eriksson J-E, Bobacka J, Boer H Bergelin M. Direct electron transfer of Trametes hirsuta laccase in a dual-layer architecture of poly(3,4-ethylenedioxythiophene) films. J Phys Chem C 2011 115 5919-5929. 

For laccase, orientation can be guided by different attachment strategies that aim specifically to attach the enzyme in a particular position [77,78]. As an example, in 2011, Pita et al. developed and optimized a strategy for oriented covalent immobilization of Trametes hirsuta laccase on gold electrodes [72]. After optimizing the immobilization for DET via the type 1 (Tl) copper site, they were able to measure current density values up to 40 pA cm for the electrocatalytic reduction of O2 in the absence of redox mediators. 

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