Multicolor detection system

Shino A, Lujioka K, Manabe N, Yamaya S, Goto Y, Yasuhara M, Yamamoto K (2005). Simultaneous multicolor detection system of the single-molecular microbial antigen with total internal reflection fluorescence microscopy. Microbiol. Immunol. 49 461 —170. 

Many researchers using the lectin microarray take advantage of a confocal fluorescence detection principle, because the method has already been adapted for established systems of DNA microarray [18, 19]. The method is simple, and has therefore been widely used. However, it requires washing of the microarray surface after the binding reaction, because a dried sample is required for the confocal-type detection system. One useful application of the system is multicolor detection using, e.g., Cy3 and Cy5, as described later. 

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