Thyroglobulin determination

Thyroid-stimulating hormone (TSH thyrotropin) In patients who have been treated surgically for thyroid carcinoma, to test for recurrence by assessing TSH-stimulated whole-body 131I scans and serum thyroglobulin determinations... 

Bioassay. Although the chemical assays described above have replaced bioassays for the determination of T and T, several m vivo and in vitro bioassays are used to determine the potency of thyroglobulin preparations and to estabUsh the thyromimetic or antithyroid potency of new compounds. 

Human MCP-1 (Fig.l) is a protein of 76 amino acids (MW 8.6 Kd). It contains four cysteines at positions 11, 12, 36 and 52, which are present as two disulfide bonds in the native form (3,4). MCP-1 belongs to a family of proteins, called CC chemokines since they contain adjacent cysteine residues at positions 11 and 12 (5,6). It is also related to another family of chemokines, referred to as the CXC family of chemokines in which the first two cysteine residues are separated by a single amino acid residue(5, 6). The disulfide bonding pattern of MCP-1 has not been determined. The disulfide bonds as shown in Figure 1 have been deduced (3), based on the disulfide bonding pattern determined for the CXC chemokines p-thyroglobulin (7) and IL-8 (8). 

Serum total T4 concentrations were initially determined indirectly, using methods that measured the amount of iodine in a protein precipitate of serum (protein-bound iodine, PBI). In addition to hormonal iodine, the PBI tests also measured iodoproteins, iodotyrosines, inorganic iodine, and thyroglobulin. More specific T4 procedures involved the measurement of hormonal iodine in either a butanol extract of a serum protein precipitate (butanol-extractable iodine) or in a purified fraction of serum (I4 by column). These methods were useful because the iodine in T4 normally accounts for 80% to 90% of all iodine in serum. Both... 

Sapin R, Gasser F, Chambron J. Recovery determination in 600 sera analyzed for thyroglobulin with a recently commerciaHzed IRMA kit. Clin Chem 1992 38 1920-1. 

Determine if there is expression of cell-specific products, cell-specific structures, and transcription factors or receptors that are unique identifiers of cell types (e.g., neuroendocrine granules, peptide hormones, thyroglobulin, PSA, prostate-specific membrane antigen, inhibin, gross cystic disease fluid protein [GCDEP], villin, uroplakin, thyroid transcription factor-1 [TTE-1] or transcription factor CDX-2). 

As stated earlier in this discussion, most non-hema-topoietic malignancies of the mediastinum should be presumed metastatic until proven otherwise. Immunohistologic analysis is only variably productive in establishing a site of origin for secondary carcinomas in this location. If determinants are found that are unassociated with PTCs, such as TTF-1, thyroglobulin, prostate-specific antigen, S-100 protein, FLAP, CA 19-9 (an enteric carcinoma marker), or CA 125 (a serosal and Mullerian tract marker),it is likely that the lesion is a metastasis. Conversely, the presence of coexpression of keratin 5/6, p63, and CD5 would, at least tentatively, appear to support a thymic origin for such a neoplasm. 

Figure 6.3 Crystal structure of the mouse acetylcholinesterase-2 gallamine homodimer complex with 30% homology of 532 residues from the C-terminal cholinesterase part of human thyroglobulin (Tg). The three-dimensional structure of mouse acetylcholinesterase homodimer complex homological to part III of human Tg in Figure 6.1 was experimentally determined at a resolution of 2.20A using X-ray diffraction (Bourne et al., 2003 ExPASy access number P21836). A model of the molecule was constructed using CAChe software (Fujitsu Ltd, Japan) according to the XYZ coordinates from Protein Data Bank file (code 1 N5M.pdb).

The molecular weight of the native enzyme is determined by analytical gel filtration chromatography using a Superose 6 [HR 10/30] column (Amersham Pharmacia Biotech). Molecular weight standards [bovine thyroglobulin (670,000),... 

Urinary iodide concentrations were determined with an iodide-selective ion electrode(iodide-electrode, radiometer, Copenhagen, Denmark)The anti-thyroglobulin and -thyroid peroxidase antibodies were determined by highly sensitive ra-dioimmuno assay. 

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