Suspension assay

Herpes and pseudorabies viruses will infect BHK Cl 3 cells in suspension. 

For herpes and pseudorabies virus this assay is similar to the 

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Matheson et al. (1978) reported the results of a battery of in vivo and in vitro assays to assess the genotoxicity of 1,1-dimethylhydrazine. Included were the Ames Salmonella microsome assay, a microbial suspension assay, mutation induction at the TK locus in L5178Y mouse lymphoma cells, stimulation of UDS in WI-38 cells, and a dominant lethal assay in mice. 1,1-Dimethylhydrazine was active in all of the tests except the dominant lethal assay. 

Seed JL. 1982. Mutagenic activity of phthalate esters in bacterial liquid suspension assays. Environ Health Perspect 45 111-114. 

Saeger, YW., Kaley, R.G, II, Hicks, O., Tucker, E.S. Mieure, J.P. (1976) Activated sludge degradation of adipic acid esters. Appl. environ. Microbiol., 31, 746-749 Seed, J.L. (1982) Mutagenic activity of phthalate esters in bacterial liquid suspension assays. Environ. Health Perspect., 45, 111-114... 

Sarrif AM, Donovan SM, Stewart JA, et al. 1983. Inhibition by semicarbazide of the mutagenic activities of formaldehyde (HCHO) and hexamethyphosphoramide (HMPA) in Salmonella typhimiiriam reverse suspension assay [Abstract]. Environ Mutagen 5 476. 

Microbial suspension assays (prokaryotic and eukaryotic organisms) Same as above plus mitotic recombination 35-40 Routine in most laboratories. 

When performing the suspension assay coupled to the S-9 mix, it is important that the test agent be tested both in the presence and in the absence of S-9. Thus, in the data presented in Table 7 (below), 2-aminofluorene in the absence of S-9 is toxic to the cell as evidenced by a slight drop in the viable count however, this effect is not indicative of a preferential inhibition of the pol Ai , strain, since the survival index is 1.02. On the other hand, in the presence of the S-9 activation mixture, there is evidence of DNA modification as shown by a survival index below 1.0 (i.e., 0.73). 

Suspension assays with Salmonella (see, for example, the forward-mutation assay described by Ames et a/. ) or Escherichia should be... 

Microparticle Encoding Technologies for High-Throughput Multiplexed Suspension Assays... 

Suspension assays using encoded microparticles have been shown to have molecular serrsitivities comparable to both ELISAs and microarrays and are realistic candidates for high throughput, low sample volume replacements for these technologies. 

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