MTT assay - suspension or monolayer cells

A Gloves and eye/face protection should be worn throughout the procedure because 

MTT is a mutagenic and toxic agent. Reference should be made to hazard codes 

Prepare an MTT stock solution of 5 mg mH (Sigma, St Louis) in phosphate-buffered saline (PBS), pH 7.5, and filter through a 0.22- x filter to sterilize and remove the small amount of insoluble residue. 

To 100 xl of cell suspension or cell monolayer in each microtitre well, add 10 pi of MTT (5 mg mH). 

Add 100 pi 0.04 M HCl in propan-2-ol to each well and mix thoroughly to dissolve insoluble blue formazan crystals. 

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