Subtractive hybridization

Matsumoto, N, Kitayama, H, Kitada, M, Kimura, K, Noda, M, and Ide, C, 2003. Isolation of a set of genes expressed in the choroid plexus of the mouse using suppression subtractive hybridization. Neuroscience 117,405-415. 

Subtractive hybridization, 73 354 Subtractive image dyes, 79 262 Subtractive instant color films, 79 296 Subtractive mixing, in color photography, 79 241-245... 

Kim M, Kim S, Kim S, Kim B-D (2001) Isolation of cDNA clones differentiaUy accumulated in the placenta of pungent pepper by suppression subtractive hybridization. Mol CeUs 11 ... 

Figure 5.13 Subtractive hybridization. (From Wang, T. et al.. Oncogene, 19,1519-1528, 2000. With permission.)...

Rebrikov, D.V., Bogdanova, E.A., Bulina, M.E. and Luk ianov, S.A. (2002) A new planarian extrachromoso-mal virus-like element revealed by subtraction hybridization. Molekuliarnaia Genetika Mikrobiologia I Virusologa (Moskva) 36, 1 002-1011. 

Suppression-subtractive hybridization (SSH) is another hybridization strategy that is being pursued to examine differential gene expression following parasite infection in the snail host. At least two major laboratories are currently engaged in using this approach towards the isolation of genes that are specifically expressed in either resistant or susceptible snails in response to infection. Initial results from these studies showed differential expression of several transcripts in a resistant (BS-90) and susceptible snail (M-line) in response to... 

Nowak, T.S., Woodards, A.C., Jung, Y., Adema, C.M. and Loker, E.S. (2004) Identification of transcripts generated during the response of resistant Biomphalaria glabrata to Schistosoma mansoni infection using suppression subtractive hybridization. Journal of Parasitology 90, 1034-1040. 

Lee SW, Tomasetto C, Sager R. 1991. Positive selection of candidate tumor-suppressor genes by subtractive hybridization. Proc Natl Acad Sci USA 88(7) 2825-2829. 

Marenholz I, Zirra M, Fischer DF, Backendorf C, Ziegler A, Mischke D. 2001. Identification of human epidermal differentiation complex (EDC)-encoded genes by subtractive hybridization of entire YACs to a gridded keratinocyte cDNA library. Genome Res 11(3) 341—355. 

Rather than describing changes in expression of a known class of genes, several studies have utilized unbiased approaches to determine changes in expression of a large number of genes in association with arousal state using cDNA arrays, DNA chips, or other molecular techniques. Subtractive hybridization was used to isolate two mRNAs whose expression was altered in forebrain after 24-hr SD... 

CSP-related proteins were independently discovered in Drosophila using subtractive hybridization and called either OS-D or A10 (McKenna et al., 1994 Pikielny et al., 1994). The proteins were expressed in subsets of olfactory hairs, consistent with a proposed function in olfaction. Additional OS-D homologs were subsequently identified based on sequence similarity (Mameli et al., 1996 Tuccini et al., 1996 Picimbon and Leal, 1999 refs listed in Table 17.1). Angeli et al. (1999) proposed the abbreviation CSP, for chemosensory protein, and this term will be used hereafter. 

Jiang, H., Lin, J. J., Su, Z. Z., Goldstein, N. L, and Fisher, P. B. (1995). Subtraction hybridization identifies a novel melanoma differentiation associated gene, mda-7, modulated during human melanoma differentiation, growth and progression. Oncogene 11, 2477-2486. 

Preliminary EST data fiom this study as well as suppression subtractive hybridization results have identified several promising gene candidates. In addition, we have recently... 

While cDNA libraries can be used to obtain many genes in a short time frame, it may not yield genes of toxicologic importance. To enrich for these genes there are several methods that rely on obtaining mRNAs that are differentially expressed after treatment. These methods, which are described below, include subtractive hybridizations, DD RT-PCR and SAGE. 

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