Subtractive hybridization high ratio

High ratio subtractive hybridization by cloning inhibition In the method described by Klickstein (1987), ( + ) cDNA with certain restriction sites (e.g., EcoRI) and a fragmented (-) cDNA library lacking these restriction sites are mixed in a proportion of 1 50, denatured and hybridized so that only (-I-) DNA unhybridized with ( -) DNA can be cloned in EcoRl-digested vectors (Fig. 12.1(1)). 

High ratio subtractive hybridization by biotin- or poly(A) -capture... 

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