Subtractive hybridization differential screening

Fig. 1. Differential screening of a human cDNA library with subtracted PolyA cDNA probes. A human cDNA library is prepared using the lambda cloning vector Lambda ZAP (Stratagene). Two replica filters are produced from a single petri dish containing approximately 2,000 recombinant clones. One filter is hybridized with a probe enriched for sequences expressed in unilineage cells (U-M), and the other hybridized with a probe enriched for sequences expressed in multilineage cells (U-M). The arrows indicate clones that were hybridized strongly with one probe and not the other.

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