Chemosensory protein

Campanacci V., Mosbah A., Bomet O., Wechselberger R., Jacquin-Joly E., Cambillau C., Darbon H. and Tegoni M.. (2001) Chemosensory protein from the moth Mamestra brassicae. Expression and secondary structure from H and 15N NMR. Eur. J. Biochem. 268, 4731 1739. 

Picimbon J. F., Dietrich K., Breer H. and Krieger J. (2000). Chemosensory proteins of Locusta migratoria (Orthoptera Acrididae). Insect Biochem. Mol. Biol. 30, 233-241. 

Campanacci V., Spinelli S., Lartigue A., Lewandowski C., Brown K., Tegoni M. and Cambillau C. (2001a) Recombinant chemosensory protein (CSP2) from the moth Mamestra brassicae crystallization and preliminary crystallographic study. Acta Crystallogr. D. Biol. Crystallogr. 57, 137-139. 

Jacquin-Joly E., VogtR. G., Francois M. C. and Nagnan-Le Meillour P. (2001) Functional and expression pattern analysis of chemosensory proteins expressed in antennae and pheromonal gland of Mamestra brassicae. Chem Senses 26, 833-844. 

Lartigue A., Campanacci V., Roussel A., Larsson A. M. and Alwyn T. (2002a) X-ray structure and ligand binding study of a moth chemosensory protein. J. Biol. Chem. 277, 32094-32098. 

Mosbah A., Campanacci V., Lartigue A., Tegoni M., Cambillau C. and Darbon H. (2002) The solution structure of a chemosensory protein (CSP) from the moth Mamestra brassicae. Biochem. J. (e-published, September). 

CSP-related proteins were independently discovered in Drosophila using subtractive hybridization and called either OS-D or A10 (McKenna et al., 1994 Pikielny et al., 1994). The proteins were expressed in subsets of olfactory hairs, consistent with a proposed function in olfaction. Additional OS-D homologs were subsequently identified based on sequence similarity (Mameli et al., 1996 Tuccini et al., 1996 Picimbon and Leal, 1999 refs listed in Table 17.1). Angeli et al. (1999) proposed the abbreviation CSP, for chemosensory protein, and this term will be used hereafter. 

Picone D., Crescenzi O., Angeli S., Marchese S., Brabdazza A., Ferrara L., Pelosi P. and Scaloni A. (2001) Bacterial expression and conformational analysis of a chemosensory protein from Schistocerca gregaria. Eur. J. Biochem. 268, 4794 -801. 

The odorant-binding proteins (OBPs) and the chemosensory proteins (CSPs) are proteins from the lymph that are thought to accomplish these tasks, solubilizing and protecting the odorant and contact chemosensory molecules. This chapter describes the biochemical and evolutionary aspects of these two families of peripheral sensory proteins of insects. Particular attention will be paid to the subclassification of binding proteins, the diversity of gene structures and the phyletic and molecular relatedness between binding proteins from different insect species. 

The family of chemosensory proteins 18.3.1 The concept of chemosensory protein... 

In the context of evolution of pheromone olfaction and general chemosensation, we can speculate that general chemosensory proteins may be more highly conserved than olfactory PBPs when compared across species. Indeed, little sequence diversification would be expected to occur among genes encoding chemosensory proteins tuned to bind chemicals of common importance to all species. 

Monteforti G., Angeli S., Petacchi R. and Minnocci A. (2002) Ultrastructural characterization of antennal sensilla and immunocytochemical localization of a chemosensory protein in Carausius morosus Bruner (Phasmida Phasmatidae). Arthrop. Struct. Dev. 30, 195-205. 

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