Strand displacement

Nielsen P.E., Egholm M., Berg R.H., Buchardt O. Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science 1991, 254 1497-1500. 

Cherny D.Y., Belotserkovskii B.P., Frank-Kamenetskii M.D., Egholm M., Buchardt O., Berg R. H., Nielsen, P.E. DNA unwinding upon strand-displacement binding of a thymine-substituted polyamide to double-stranded DNA. Proc. Natl Acad. Sci. USA 1993 90 1667-1670... 

Both target and signal amplification systems have been successfully employed to detect and quantitate specific nucleic acid sequences in clinical specimens. Polymerase chain reaction (PCR), nucleic acid sequence-based amplification (NASBA), transcription-mediated amplification (TMA), strand displacement amplification (SDA), and ligase chain reaction (LCR) are all examples of enzyme-mediated, target amplification strategies that are capable of producing billions of... 

Walker, G. T., et al. (1992). Strand displacement amplification—an isothermal, in vitro DNA amplification technique. Nucleic Acids Res. 20, 1691-1696. 

In summary, two different Gain-of-Interaction models have been proposed for the fibrillar structure of /12m. The cross-(3 spine model (Ivanova et al., 2004) proposes a core composed of C-terminal /1-hairpins, and the direct-stacking model (Benyamini et al., 2003) proposes a core of native-like /12m molecules with their N- and C-terminal strands displaced. 

HCV and HIV-1). The bDNA assay is being much employed for the quantification of messenger RNA. Moreover, for the detection of viral and pathogenic disorders based on alkahne-phosphatase-sensitive dioxetanes, several assay methods are available these include the Polymerase-Chain-Reaction (PCR) amphfication, probe ligation, strand-displacement amplification and the ligase chain reaction. ... 

Kong, H., R.B. Kucera and W.E. Jack 1993. Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. J. Biol. Chem. 268 1965-1975. 

Figure 2. R loops formed by mRNA from a K-producing plasmacytoma hybridized to DNA from the same tumor. An R loop is formed when mRNA hybridizes to the complementary DNA strand, displacing the sense DNA strand. In this example, two R loops are formed, one containing C and the other V region sequences. These two loops are separated by a double-stranded intron of -3.7 kb. The tail extending from the C region R loop presumably consists of the poly(A) segment of the mRNA. (Photograph reproduced with permission from Seidman and Leder, 1978 diagram modified from same figure.)...

Kurakin, A., Larsen, H.J. and Nielsen, P.E. (1998) Cooperative strand displacement by peptide nucleic acids (PNA). Chem. Biol., 5, 81-89. 

Mollegaard, N.E., Buchardt, O., Egholm, M. and Nielsen, P.E. (1994) Peptide nucleic acid. DNA strand displacement loops as artificial transcription promoters. Proc. Natl. Acad. Sci. USA, 91,3892-3895. 

In another report, the DNA amplification was based on strand displacement amplification (SDA). This method worked at an isothermal temperature of 50°C for the 106-bp product from Mycobacterium tuberculosis [942]. 

The biochemical process of recombination occurs by breaking and rejoining DNA strands. The key reaction is strand displacement initiated at a nick in the chromosome. Then a protein called RecA (which stands for recombination rec bacteria are unable to recombine their DNA information and therefore are abnormally sensitive to UV radiation) binds to a single-stranded DNA fragment and catalyzer its exchange with the same sequence of the duplex. RecA protein is a strand displacement protein. See Figure 8-16. 

RecA preferentially binds to single-stranded DNA in a cooperative fashion this cooperativity means that RecA will cover an entire single-stranded DNA molecule rather than bind to several molecules partially. Rec A then aligns homologous segments (those with complementary information) to form base pairs. The key reaction of RecA-coated DNA is the movement of the single-stranded regions of the DNA to form a joint molecule—a process called strand displacement. This reaction involves ATP hydrolysis. 

Rolling circle amplification, an isothermal method of amplifying circular DNA using strand displacement Smart strept(avidin)s A strept(avidin) that responds to its environment SMPB Succinimidyl 4-[malemidophenyl] -butyrate... 

Peptide nucleic acids (PNAs) are oligonucleotide-like molecules that have their DNA backbone removed and replaced with an achiral polyamide backbone that can hybridize with DNA through strand displacement (58). PNA tags have been used in the development of tagged libraries that allow for the spatially addressable localization and identification of probes on a DNA microarray. 

Nielsen PE, Egholm M, Berg RH, Buchardt O. Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide. Science 1991 254 1497-1500. 

Strand displacement amplification (SDA) Target Hindi DNA polymerase I (exonuclease deficient) No... 

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