Rolling circle amplification

This problem can also be lessened by using stronger fluorochromes and chemiluminescent substrates for use in ELISAs, immunofluorescence-based staining, and immunoblotting. 

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Kuhn H., Demidov V.V., Frank-Kame-NETSKii M.D. Rolling-circle amplification under topological constraints. Nucleic Acids Res. 2002 30 574-580... 

Regression correlation coefficient Regression coefficient of determination Rolling circle amplification Water solubility Sodium dodecyl sulfate Supercritical fluid extraction Standard operating procedure Solid-phase extraction Surface plasmon resonance Thymine... 

Many nucleic acid detection strategies use target amplification, signal amplification or both. Invader, branched DNA (bDNA) and rolling circle amplification (RCA) are three approaches. 

Rolling circle amplification (RCA) is an alternative method to polymerase chain reaction it is also a generic amplification technique that can be used in antibody assays. Using a replication process similar to that used by viruses, RCA allows the recognition,... 

Since rolling circle amplification takes place at a constant temperature, there is no need for the target amplification process to take place in a thermal cycler, which is required to regulate the temperature for different parts of the reaction. The type of DNA polymerase to be used in RCA is not limited to thermostable enzymes, like the PCR-based diagnostics. On the other hand, the RCA method requires the environment to be free of contaminations as the RCA arrays are highly sensitive. Wiltshire [22]... 

D.C. Thomas, G.A. Nardone, and S.K. Randall, Amplification of padlock probes for DNA diagnostics by cascade rolling circle amplification or the polymerase chain reaction. Arch. Pathol. Lab. Med. 123, 1170-1176 (1999). 

S. Wiltshire, S. O Malley, J. Lambert, K. Kukanski, D. Edgar, S.F. Kingsmore, and B. Schweitzer, Detection of multiple allergen-specific IgEs on microarrays by immunoassay with rolling circle amplification. Clin. Chem. 46,1990-1993 (2000). 

Figure 1.16 Critical issues facing DNA microarrays and protein microarrays. MIAME = minimum information about a microarray experiment, checklist endorsement as of October 14, 2002. RCA = rolling circle amplification. RLS = resonance light scattering.

Note ELF = enzyme labeled fluorescence. ECL = enhanced chemiluninescence. CL = chemiluminescence. RES = biotin amplification, sAV-Cy3. RCA = rolling circle amplification. 

Lizardi, P.M., Huang, H., Zhu, Z., Bray-Ward, P, Thomas, D.C., and Ward, D.C., Mutation detection and single-molecule counting using isothermal rolling circle amplification, Nat. Genetics, 19, 225-232, 1998. 

A novel signal amplification method based on the rolling circle amplification (RCA) technology has also been reported. RCA involves many rounds of isothermal enz5miatic... 

Nallur G, Luo C, Fang L et al. Signal amplification by rolling circle amplification on DNA microarrays. Nucleic Acids Res 2001 29 E118. 

Mullenix MC, Dondero RS, Datta HD, Egholm M, Kingsmore SF, Perlee LT. Rolling circle amplification in multiplex immunoassays. In Demidov W, Broude NE, editors. DNA Amplification—Current Technologies and Applications. Norfolk, UK Horizon Bioscience, 2004 313-331. 

Kingsmore SF, Patel DD Multiplexed protein profiling on antibody-based microarrays by rolling circle amplification. Curr. Opin. Biotechnol. (2003) 14 74—81. 

Rolling circle amplification, an isothermal method of amplifying circular DNA using strand displacement Smart strept(avidin)s A strept(avidin) that responds to its environment SMPB Succinimidyl 4-[malemidophenyl] -butyrate... 

Fig. 9 d In rolling circle amplification, a circular template was replicated continuously. A double-stranded DNA was used to hold the reactive above the surface... 

The one-base extension method [73], rolling circle amplification (RCA) method [74]and Taqman PCR method [75], have been applied to SNP detection. Also a DNA chip which anchored oligonucleotide on the substrate has been developed as a simple hybridization detection tool [76-82], For SNP detection, it is not easy to determine the difference of one base mismatch with this hybridization detection method due to unstable hybridization between probe and SNP and nonspecific adsorption. 

Shao, W., Wang, M., Fu, Q., Shu, Q., Laroche, L, Zhou, Z., Tchernev, V.T, etal. (2002). Multiplexed protein profiling on microarrays by rolling-circle amplification. Nat. Biotechnol. 20,... 

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