RecA protein

Belotserkovskii B.P., Zarling D.A. Peptide nucleic acid (PNA) facilitates multistranded hybrid formation between linear double-stranded DNA targets and RecA protein-coated complementary single-stranded DNA probes. Biochemistry 2002 41 3686-3692. 

The bottom-up synthesis of metallic nanowires was further applied to construct a nanotransistor device.93 The sequence-specific winding of the homologous nucleic acid carried by the RecA-protein into the duplex DNA was used to address the nucleic acid/protein complex on the DNA scaffold (Fig. 12.27). The subsequent association of the anti-RecA antibody to the protein DNA complex, followed by the association of the biotinylated antiantibody, and the linkage of streptavidin-modified carbon nanotube deposited the tubes in the specific domain of the DNA scaffold. The further... 

In addition to DNA polymerase V, translesion replication requires the RecA protein, SSB, and some subunits derived from DNA polymerase III. Yet another DNA polymerase, DNA polymerase IV, is also induced during... 

Encodes protein that inhibits cell division, possibly to allow time for DNA repair Encodes RecA protein, required for error-prone repair and recombinational repair Encodes DNA polymerase IV... 

Enzymes that promote various steps of homologous recombination have been isolated from both prokaryotes and eukaryotes. In E. coli, the recB, recC, and recD genes encode the RecBCD enzyme, which has both he-licase and nuclease activities. The RecA protein pro-... 

FIGURE 25-34 RecA. (a) Nudeoprotein filament of RecA protein on single-stranded DNA, as seen with the electron microscope. The stri-ations indicate the right-handed helical structure of the filament, (b) Surface contour model of a 24-subunit RecA filament. The filament has six subunits per turn. One subunit is colored red to provide perspective (derived from PDB ID 2REB). 

RecA protein binds to single-stranded or gapped DNA. The complementary strand of the linear DNA pairs with a circular single strand. The other linear strand is displaced (left) or pairs with its complement in the circular duplex to yield a Holliday structure (right). 

Lusetti, S.L. Cox, M.M. (2002) The bacterial RecA protein and the recombinational DNA repair of stalled replication forks. Annu. Rev. Biochem. 71, 71-100. 

In the SOS system, multiple unlinked genes repressed by a single repressor are induced simultaneously when DNA damage triggers RecA protein-facilitated autocatalytic proteolysis of the repressor. 

Reading frames in nucleic acids 236, 237 Rearrangement reactions 527, 530, 712 recA protein 219 Receptor(s) 1,553-563 acetylcholine 422 alpha adrenergic. See a-Adrenergic receptors... 

Figure 27-24 Structures of RecA protein spiral filaments. (A) RecA protein filament formed on circular duplex DNA in the presence of ATP(Y-S), shadowed with Pt and seen by electron microscopy. (B) Diagram of RecA bound to duplex DNA in the presence of ATP(y-S), as determined by electron microscopy. RecA monomers are shown as spheres, but their exact shape is unknown. (C) Diagram of RecA spiral filament in crystals of RecA protein free of DNA, based on X-ray crystallographic data. Arrows indicated alignment of monomers. From Howard-Flanders, West, and Stasiak.536...

In related reactions a single-stranded SSB-coated circular DNA will bind to RecA protein, then exchange strands with a linear duplex (Eq. 27-12). The strand exchange requires ATP and advances in the 5 to 3 ... 

Figure 27-25 A possible mechanism for homologous pairing of an ssDNA with a duplex DNA and strand exchange. The ssDNA (right) binds together with a hydrogen-bonded duplex (left). The RecA protein rotates the bases into the heteroduplex configuration, where hydrogen bonds may be formed in many of the base pairs. After Howard-Flanders, West, and Stassiak.536...

Ikeda, M., Hamano, K., and Shibata, T (1992) Epitope mapping of anti-recA protein IgGs by region specified polymerase chain reaction mutagenesis. J. Biol Chem 267,6291-6296... 

Reactions catalyzed by purified recA protein in vitro. RecA catalyzes a number of different reactions between DNA strands, all of them involving the unwinding and winding of base-paired structures, (a) D-loop formation by interaction between supercoiled circular duplex DNA and single-stranded DNA. (b) Strand exchange between a gapped circular duplex structure and a linear duplex structure, (c) Complex formation between two helices, one of which is gapped. 

The fact that mutations in recA completely eliminate recombination, as well as the finding that the recA-related protein catalyzes strand exchange reactions like those required in the recombination models we have been discussing, makes it highly likely that recA plays a key role in these processes in vivo. If this is so, then recA protein is clearly situated at the hub of activities in the recombination complex. 

What is the role of the recA protein in E. coli DNA repair and in recombination What use are recA mutants in biochemical and genetic research ... 

The recA protein is very important in DNA repair. An insult to the DNA leads to the activation of the... 

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