Isothermal strand displacement amplification

Another example of a DNA machine that can easily be triggered by the actions of an aptamer is based on the isothermal strand displacement amplification (Weiz-mann et al., 2006b). In this case, a single-stranded DNA track contains three distinct domains domain I for the binding of a primer DNA to start replication domain II, which can, upon dnplex formation, be recognized by a nicking endonuclease (which cuts one strand of a double-stranded structure) and domain... 

Walker, G. T., et al. (1992). Strand displacement amplification—an isothermal, in vitro DNA amplification technique. Nucleic Acids Res. 20, 1691-1696. 

In another report, the DNA amplification was based on strand displacement amplification (SDA). This method worked at an isothermal temperature of 50°C for the 106-bp product from Mycobacterium tuberculosis [942]. 

Another isothermal amplification technique is strand displacement amplification (SDA). " After heat denaturation of DNA in the presence of four primers, dCTP, dGTP dUTP, and a modified deoxynucleotide (dATPaS), two enzymes are added, an exonuclease-deficient polymerase and a restriction enzyme. The two flanking primers that enter into exponential amplification have a restriction site added to their 5 end and get nicked by the restriction enzyme, allow-ing displacement of strands that can in turn be primed, extended, and nicked. Deoxy-ATPotS is used so that the restriction sites include a hemiphosphorothioate linkage to allow single-strand nicking, instead of cutting through double strands. 

He, Y, Zeng, K., Zhang, X. et al. (2010) Electrochemistry communications ultrasensitive electrochemical detection of nucleic acid based on the isothermal strand-displacement polymerase reaction and enzyme dual amplification. Electrochem. Commuru, 12 (7), 985-988. 

Rolling circle amplification, an isothermal method of amplifying circular DNA using strand displacement Smart strept(avidin)s A strept(avidin) that responds to its environment SMPB Succinimidyl 4-[malemidophenyl] -butyrate... 

Instead of specific amplification of one target to improve sensitivity, methods that amplify all genomic DNA or mRNAs are useful when the target is in short supply. For example, multiple-displacement amplification uses exonuclease-resistant random hexamers and a highly pro-cessive polymerase to amplify DNA nonspecificaily. Initial DNA denaturation is not necessary and the reaction proceeds isothermally. Similarly, messenger RNA can be generi-caUy amplified with a poly(T) primer modified with an RNA polymerase promoter. After reverse transcription, second-strand DNA synthesis, and transcription, antisense RNA is produced. Both whole genome and antisense RNA amplification are also useful as nucleic acid purification methods before amplification or detection. 

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