Strand displacement amplification

Both target and signal amplification systems have been successfully employed to detect and quantitate specific nucleic acid sequences in clinical specimens. Polymerase chain reaction (PCR), nucleic acid sequence-based amplification (NASBA), transcription-mediated amplification (TMA), strand displacement amplification (SDA), and ligase chain reaction (LCR) are all examples of enzyme-mediated, target amplification strategies that are capable of producing billions of... 

Walker, G. T., et al. (1992). Strand displacement amplification—an isothermal, in vitro DNA amplification technique. Nucleic Acids Res. 20, 1691-1696. 

HCV and HIV-1). The bDNA assay is being much employed for the quantification of messenger RNA. Moreover, for the detection of viral and pathogenic disorders based on alkahne-phosphatase-sensitive dioxetanes, several assay methods are available these include the Polymerase-Chain-Reaction (PCR) amphfication, probe ligation, strand-displacement amplification and the ligase chain reaction. ... 

In another report, the DNA amplification was based on strand displacement amplification (SDA). This method worked at an isothermal temperature of 50°C for the 106-bp product from Mycobacterium tuberculosis [942]. 

Strand displacement amplification (SDA) Target Hindi DNA polymerase I (exonuclease deficient) No... 

Another isothermal amplification technique is strand displacement amplification (SDA). " After heat denaturation of DNA in the presence of four primers, dCTP, dGTP dUTP, and a modified deoxynucleotide (dATPaS), two enzymes are added, an exonuclease-deficient polymerase and a restriction enzyme. The two flanking primers that enter into exponential amplification have a restriction site added to their 5 end and get nicked by the restriction enzyme, allow-ing displacement of strands that can in turn be primed, extended, and nicked. Deoxy-ATPotS is used so that the restriction sites include a hemiphosphorothioate linkage to allow single-strand nicking, instead of cutting through double strands. 

Walker GT, Linn CP, Nadeau JG. DNA detection by strand displacement amplification and fluorescence polarization with signal enhancement using a DNA binding protein. Nucleic Acids Res 1996 24 348-53. 

Another example of a DNA machine that can easily be triggered by the actions of an aptamer is based on the isothermal strand displacement amplification (Weiz-mann et al., 2006b). In this case, a single-stranded DNA track contains three distinct domains domain I for the binding of a primer DNA to start replication domain II, which can, upon dnplex formation, be recognized by a nicking endonuclease (which cuts one strand of a double-stranded structure) and domain... 

Figure 4.8 DNA machine based on strand displacement amplification. The track consists of a primer binding sequence (black, I), a nicking recognition sequence (green, II), and a template for the product DNA (blue, IE). (1) Binding of the primer starts replication of the entire track by a DNA polymerase. (2) The fully replicated duplex is nicked on the newly formed strand by the nicking enzyme, regenerating the primer site...

Figur 4 8 DNA machine based on strand displacement amplification. S66 text for full description.)...

In addition to PCR, there are many other technologies to amplify nucleic acids. For example, ligation-based amplification or ligase chain reaction uses sequence-directed oligonucleotide primers and thermostable DNA ligase to assay point mutations, deletions, or insertions in DNA. Strand-displacement amplification uses the inherent strand-displacement activity of DNA polymerases to conduct DNA amplification at a constant temperature. Transcription-based methods such as nucleic acid sequence-based amplification (NASBA) involve in vitro RNA transcription. NASBA and most other transcription-based... 

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