Strain construction

Assuming that a diamond-pyramid hardness test creates a further nominal strain, on average, of 0.08, and that the hardness value is 3.0 times the true stress with this extra strain, construct the curve of nominal stress against nominal strain, and find ... 

A comprehensive list of behavioral phenomena and physical attributes affecting the strength and stability of steel frames is compiled in White 1991. Some of the items listed include initial imperfections, residual stresses, initial strains, construction sequence, effects of simultaneous axial force, shear and moment on section capacities, P-dclta effect, local buckling and spread of inelastic zones in members. A similar list of items could be compiled for reinforced concrete and other structural materials. It is clear that a comprehensive advanced analysts can become quite... 

Oligonucleotide Primers Used in Plasmid and Strain Construction... 

Aarnikunnas et aL (2002) used metabolic engineering of L. fermentum for the production of mannitol and pure L-lactic acid or pyruvate. The authors first developed genetic tools to modify L. fermentum and then proceeded to inactivate first IdhD gene and then IdhL gene to create a bacterium that could produce mannitol and either pure L-lactic acid or pyruvic acid in a single process. In bioreactor cultivations, the single mutant strain constructed by... 

Fonzi WA, Irwin M Isogenic strain construction and gene mapping in Candida albicans. Genetics 1993 134 717-728. 

Figure 5. Dendrogram of MLVA of 17 F. tularensis strains constructed using all 25 VNTR marker loci and the programs FreeTree and TreeView.

Two basic types of assays were used. Lethality studies compared the lethal effect of compounds on wild type bacteria Ea-aheviohia ooli) K-12 with the lethality on several mutant strains constructed with known DNA repair deficiencies. Compounds which are more toxic to such a repair deficient mutant than to the wild type are considered to be genetically toxic. Table I lists the bacterial strains used, together with their genotypes. 

Strain Construction To Produce PDO from Glucose Directly... 

Graph of stress as a function of strain constructed from data obtained in any mechanical test in which a load is apphed to a material and continuous measurements of stress and strain are made simultaneously. It is constructed for tensile, creep, or torsional loadings. 

Recent technical advances in strain/pathway construction have moved the limiting step from strain construction to screening. Those advances are, among others, affordable gene synthesis, synthetic construction of gene libraries (Collier et al., 2009 Bott et al., 2012), and novel techniques for DNA assembly (e.g., Engler et al., 2008 Gibson et al., 2009). 

Application of systans metabolic engineering gives rise to breakthroughs in strain construction. So far, a few E. coli L-threonine hyper-producers have been constructed by this means, and the total conversion rate of glucose to L-threonine and biomass approximates to the predicted theoretical values. 

Todd, R.B., Davis, M.A., Hynes, M.J., 2007a. Genetic manipulation olAspergilus nidulans meiotic progeny for genetic analysis and strain construction. Nature Protocols 2,811-821. 

Bacterial and eukaryotic cell growth conditions and strain construction. For details see Supplementary Methods online. 

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