Gene libraries

An effective and generic method of screening of BVMO activity is indispensable for screening metagenome libraries as these libraries are often in the range of 100000-1000000 clones. Therefore, it is a challenge to develop a novel way to efficiently detect BVMO activity in gene libraries. This would facilitate... 

Marks, J. D., Hoogenboom, H. R., Bonnert, T. P., McCafferty, J., Griffiths, D., and Winter, G. (1991). By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222, 581-597. 

Joern, J.M., Meinhold, P. and Arnold, F.H. (2002) Analysis of shuffled gene libraries. Journal of Molecular Biology, 316, 643-656. 

This question can be tackled with a concrete research project, which started recently in my group. The basic idea is to make a large number of NBPs 50 residues long. The maximum theoretical number of such chains is 20, and by using the well-known technique of phage display, gene libraries containing approximately 10 clones can be produced. 

This method is often used in screening gene libraries made from sheared genomic DNA or from cDNA. There is, however, the technical difficulty of deciding on the sequence to be used in the probe, if the cloned gene s sequence is not yet known. Fortunately, there are some ways around this problem. 

To circumvent some of the limitations of direct immunization, phage display technology has been applied to the preparation of fully human MABs. Gene libraries of cDNA from nonimmune or immunized donor lymphocytes are expressed in bacteriophages. The bacteriophages display functional antibody fragments and can... 

De Wildt, R.M.T., Finnem, R., Ouwehand, W.H., Griffiths, A.D., Van Venrooy, W.J., and Hoet, R.M.A. (1996). Characterization of human variable domain antibody fragments against the U1 RNA-associated A protein, selected from a synthetic and a patient derived combinatorial V gene library. Eur. J. Immunol., 26, 629-639. 

J. Perez-Ortin, M. Del Olmo, E. Matallana, and V. Tordera, Biocbem. Educ. 25, 237-242 (1997). Making Your Own Gene Library. ... 

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