Stains trays

Alternatively, the waste volume can be minimized by the addition of paper tissue to one corner of the staining tray. Coomassie brilliant blue is removed from the gel without changing the destaining solution, minimizing the waste volume generated. The tissues are replaced when they are saturated with Coomassie brilliant blue. Caution should be used, however, because excessive destaining will lead to loss of band intensity. 

NOTE Clean all equipment used for running and staining the gel with detergent and rinse thoroughly. Wear clean gloves when handling the electrophoresis apparatus, the gel, or the staining tray. 

Equipment essential for electrophoresis includes power sources, gel molds, sample grinding trays, electrode buffer trays, reusable ice packs (or a 4° cold room), a gel slicer, staining trays, assorted glassware, variable temperature incubators), hot/stir plates, and a pH meter. Details on equipping a laboratory for electrophoresis are given elsewhere.12,17... 

Gently rock the staining trays on occasion to ensure that the stain buffer is evenly distributed over the gel. Gels should be incubated in the dark at 37° unless otherwise noted. Monitor the gels closely and stop the reaction (by pouring off the stain solution and adding water or fixative) when the resolution of the bands is optimal. 

Stain the gel in the dark at room temperature keep the staining tray tightly covered ... 

Transfer the gel to the Gel Staining Tray to stain the gel for DNA Visualization with Methylene Blue Plus. 

Ethidium bromide Use 0.5 pg/mL solution. Amount varies depending on size of staining trays/ gels, roughly 50 mL/gel. Store at room temperature. EtBr stain is reusable. Please handle EtBr stain safely. 

Heat blocks or water baths Microfuge racks Permanent markers Staining trays... 

Race the agarose gel in a staining tray. Pour enough ethidium bromide (0.5pg/mL) to cover the gel. Wait 20 minutes. 

Pour the ethidium bromide solution back into its storage bottle. Pour eitough wrater into the staining tray to cover the gel. Wait 5 minutes. Pour the water out of the staining tray into a hazardous waste contairrer and place the... 

Carefully remove the gel from the plastic or glass plates using clean gloves and transfer it into a clean staining tray containing distilled water. 

The principal application of melamine-formaldehyde moulding compositions is for the manufacture of tableware, largely because of their wide colour range, surface hardness and stain resistance. The stain resistance does, however, leave something to be desired and one aim of current research is to discover alternative materials superior in this respect. Cellulose-filled compositions also find a small outlet for trays, clock cases and radio cabinets and other purposes. The mineral-filled powders are used in electrical applications and knobs and handles for kitchen utensils. 

Remove the gel, carefully place it in a tray or large watch glass, and cover it with methylene blue. Wear gloves when handling stain. Stain for at least 30 min. Overnight is fine. 

Transfer the gel gently into a tray containing staining solution. 

Fluid that is flushed from the capillary gap is collected in a waste tray beneath the slide-coverplate assemblies. This tray has a limited capacity and must be drained prior to a staining run. The entire slide-coverplate and reagent carousel area are enclosed within a Plexiglascover during operation, which protects reagents from laboratory conditions (temperature, humidity) and prevents inadvertent contact with the robotic pipet arm during movement. 

Place the slide into a tray, stain with Soln. C for 5-10 min, and discolor with Soln. B until the background is colorless. 

The specimens are placed by litter in stainless steel trays, transferred into the processor, and stained in alcian blue stain for 48 h. The alcian blue stain is then replaced with ethanol. After decoloration and dehydration for 72 h the ethanol is decanted and replaced with 1% KOH solution for 48 h. The actual staining step follows... 

Run the slab gel in a vertical dimension. The current should be set at 25-30 mA per slab gel. Allow the electrophoresis to proceed until the tracking dye is at the bottom of the gel. Turn the power off and remove the gels from the buffer chambers. Carefully separate the sandwiched plates with a thin spatula and transfer the gel in one piece to a tray containing staining solution. 

Trays for staining, blotting, and incubation III. EXPERIMENTAL PROCEDURE... 

Add just enough Coomassie brilliant blue staining solution so that the gel floats freely in the tray. Shake slowly 4 hr to overnight on a laboratory shaker or rocker. 

Disassemble gel cassettes, place gels in separate covered trays, and immerse one gel in 50 ml of 2% casein in 50 mM Tris-Cl for 30 min at 4°C. Stain second gel with Coomassie brilliant blue as described (see Support Protocol 1, steps 1 to 5). 

A plain hypo bath is often used prior to toning and sometimes as the second bath in a two-bath system, ft has a short tray life and is not efficient at neutralizing alkali brought over from the developer. Used as the first bath with paper (Fixing Paper, below), or as the primary bath for film, a plain hypo bath may cause stains and other problems. For these reasons, it is not considered suitable for general applications or as a first bath. 

Store the solution in a stoppered glass bottle away from light. Pour a small volume of TC-1 into the tray or vessel to be cleaned. Slosh it around so that the solution has access to all parts of the tray then pour the solution out and wash the tray thoroughly with water until all traces of the cleaner disappear. This solution will remove stains caused by oxidation products of developers as well as some silver and dye stains. It should not be used to clean hands. 

To remove stains in trays from silver, silver sulfide, and many dyes, pour a small quantity of Solution A into the tray and allow to remain for a few minutes rinse well and then replace with a similar volume of Solution B. Agitate to clear the brown stain completely then wash thoroughly. 

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