Silver nitrate stain

In ammoniacal solution (in which the ion [AgfNHjlj]" is formed) it is readily reduced to silver (see above) by many organic compounds. The use of silver nitrate for marking clothes depends on its reduction by the material to black silver. The reduction also occurs even when the neutral solution comes in contact with the skin, and a black stain is left. Thus solid silver nitrate rubbed on the skin leaves a black deposit and so is used in surgery as a mild caustic—hence the old name for silver nitrate of lunar caustic. 

Silver nitrate solution is highly toxic and will stain skin or clothing. 

Silver nitrate is corrosive and will stain skin and clothing. 

Silver nitrate is caustic, highly toxic, and will stain skin. 

Cover photo Tissue print of okra fruit stained with acidic silver nitrate for ascorbic acid localization. Photo courtesy of Dr. Rosannah Taylor. 

In 2007, Dickson et al. found that it is possible to stain fixed cells with fluorescent silver clusters instead of silver nanoparticles by tuning the staining conditions [57]. The new approach consists of staining fixed cells with a low concentrated silver nitrate solution 20-100 mM, within 20 h at ambient conditions, and reducing the silver by photoactivation, with the result of small silver clusters that present a broad emission band between 500 and 700 nm (Fig. 8a-d). The discovery that fluorescent silver clusters can be generated by photoactivation of cells fed with silver salt, opens up new paths for the application of silver clusters in biological systems. 

Fig. 10 (a) Fluorescence image of methanol-fixed NIH3T3 cells loaded with peptide encapsulated silver clusters for 1 h at room temperature, (b) Time profile of the time series images of cell stained with silver nitrate showing the fast silver cluster emission centered in the nucleus at short times with a maximum at 320 nm. Note that black indicates an intermediate intensity level in this color scheme [57]... 

Silver nitrate, which is a caustic agent, is available as a stick or pen in combination with potassium nitrate and is suitable for the removal of warts on the hands and feet. It should be used with caution and patients are advised to protect the surrounding skin, as it can cause chemical burns. It can also cause staining of skin and fabric. 

Its action is similar to that of silver nitrate but it causes sharp pain and does not stain the dentine. 

A yellow to brown or black stain is produced,4 which is compared with a set of standard strips prepared under similar conditions. The chief difficulty encountered is to obtain a reliable and permanent set of standards especially is this the case with silver nitrate, the stains of which do not keep. The most satisfactory method5 of preparing such stains is to soak the filter paper in gum tragaeanth, dry it, soak it in silver nitrate solution, again dry it, expose to arsine under the requisite conditions and fix the stain by repeated soaking in very dilute ammonia and coating with paraffin. By the use of 66 per cent, silver nitrate solution, OT x 10-6 g, of As may be detected.6... 

After an electrophoresis run is complete, the gel must be analyzed to answer analytical or experimental questions. As most proteins are not directly visible, the gel must be processed to determine the location and amount of the separated proteins. The most common analytical procedure is staining. Proteins are usually stained with Coomassie brilliant blue or silver nitrate. 

Hirai, H. (1988) Paragonimus ohirai identification of nucleolar organizer regions (NORs) and silver nitrate staining pattern in spermatogenesis. Experimental Parasitology 67, 281-286. 

In the present case, both the Coomassie and the more sensitive silver nitrate stain yield the same results. After the last step, the target protein, (R)-ADI I, can be regarded as homogeneous. 

Perchloric acid and paper towels were used to clean up a silver nitrate stain. After placing in a waste bin, the towels ignited. 

Find the spots on the gel corresponding to landmarks on the film (see Note 26) and modify the size of the silver nitrate image adjusting it to the smaller one of film by the mean of adequate software. Actually the gel is larger than the film owing to silver staining procedure. 

Silver nitrate (AgNO ) Demo 4.1 Stains and bums skin. Irritates mucous membranes. Can cause blindness if splashed in eyes. Wear plastic gloves, goggles. 

The eluted GFPuv samples were run on a 12% SDS-PAGE gel along with samples of TPP-extracted GFPuv. The first 30 min was performed at a fixed voltage of 50 V, which was increased to 200 V for the last 40 min. The protein bands were visualized with Coomassie Brilliant Blue. The same samples were nm on another 12% SDS polyacrylamide gel at a fixed voltage of 350 V (approx 1 h) and stained with silver nitrate solution, using the PhastSystem (Amersham Biosciences). 

Fig. 2. Polyacrylamide gel run by SDS-PAGE (PhastSystem). Protein was stained with silver nitrate. Gel visualization was altered by nitrate oxidation. The eluted samples from HIC columns applied to the wells were as follows lane 1, mass molecular weight markers, 18-200 kDa (Gibco) lane 2, sample eluted from methyl column (0.085 pg) lane 3, sample eluted from butyl column (0.080 pg) lane 4, sample eluted from octyl column (0.077 pg) lane 5, sample eluted from phenyl column (0.069 pg).

Caution Silver nitrate stains skin and clothing. Wear rubber gloves. Should staining result, try removal with one of the stain removers under Formulas Miscellaneous. 

Stain the gel for 10 min with freshly prepared staining solution prepared as shown below Mix 4 mL of concentrated ammonium hydroxide with 56 mL of 0.1 M sodium hydroxide. Add 200 mL water, and 10 ml of 20% (w/v) silver nitrate in drops with constant stirring. The final volume is adjusted to 300 mL with water. 

Proteins are stained with 0.03% (w/v) silver nitrate in deionized water followed by developing with a solution of 10% saturated aqueous Na2C03 solution containing 0.1% (v/v) of saturated aqueous formaldehyde (40% [v/v]). Development is terminated by acetic acid (3% [w/v] in water) (see Note 16). 

Stain proteins with silver nitrate solution (see Note 28). 

Nascent hydrogen, from zinc and sulphuric acid, reduces both acids to phosphine, which may be detected by the yellow stain which it produces on silver nitrate —... 

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