Silver nitrate staining

Hirai, H. (1988) Paragonimus ohirai identification of nucleolar organizer regions (NORs) and silver nitrate staining pattern in spermatogenesis. Experimental Parasitology 67, 281-286. 

In the present case, both the Coomassie and the more sensitive silver nitrate stain yield the same results. After the last step, the target protein, (R)-ADI I, can be regarded as homogeneous. 

Perchloric acid and paper towels were used to clean up a silver nitrate stain. After placing in a waste bin, the towels ignited. 

Caution Silver nitrate stains skin and clothing. Wear rubber gloves. Should staining result, try removal with one of the stain removers under Formulas Miscellaneous. 

Safety warning Silver nitrate stains skin and clothing. Use gloves when working with this reagent, and clean all spills with water. 

The damages that occur during various pre-treatment processes of cellulosic fibres can be detected by various chemical tests like fluidity, copper number. Methylene Blue absorption and silver nitrate staining etc. 

Silver nitrate stains the fingers. Clean hands the day after this experiment are a sign of excellent technique. 

P40 monomer and dimer spots were assigned on the silver nitrate stained... 

Figure 14. 2-D PAGE analysis of P40. One hundred and fifty rg of P40 (batche 3) were loaded onto a 5-8 IPG strip by in-gel rehydration. After focusing, proteins were separated in the second dimension on a 15% homogeneous gel before (A) transfer to a PVDF membrane and immunoblotting with the rabbit anti-HCP serum, or (B) silver nitrate staining.

The two main procedures for microspread staining are (1) phosphotungstic acid (PTA) staining and (2) silver nitrate staining. 

Fig. 10. MC3T3-E1 cells cultured on a PLLA scaffold with parallel open microtubular architecture for 4 weeks in vitro, von Kossa s silver nitrate staining. Reprinted from Ref. 111. 2001, by permission of John Wiley Sons.

CAUTION Silver nitrate stains the skin. Protective gjoves should be worn during this operation. The —10% silver nitrate/silica gel used in this separation is commercially available. 

In ammoniacal solution (in which the ion [AgfNHjlj]" is formed) it is readily reduced to silver (see above) by many organic compounds. The use of silver nitrate for marking clothes depends on its reduction by the material to black silver. The reduction also occurs even when the neutral solution comes in contact with the skin, and a black stain is left. Thus solid silver nitrate rubbed on the skin leaves a black deposit and so is used in surgery as a mild caustic—hence the old name for silver nitrate of lunar caustic. 

Silver nitrate solution is highly toxic and will stain skin or clothing. 

Silver nitrate is corrosive and will stain skin and clothing. 

Silver nitrate is caustic, highly toxic, and will stain skin. 

Cover photo Tissue print of okra fruit stained with acidic silver nitrate for ascorbic acid localization. Photo courtesy of Dr. Rosannah Taylor. 

In 2007, Dickson et al. found that it is possible to stain fixed cells with fluorescent silver clusters instead of silver nanoparticles by tuning the staining conditions [57]. The new approach consists of staining fixed cells with a low concentrated silver nitrate solution 20-100 mM, within 20 h at ambient conditions, and reducing the silver by photoactivation, with the result of small silver clusters that present a broad emission band between 500 and 700 nm (Fig. 8a-d). The discovery that fluorescent silver clusters can be generated by photoactivation of cells fed with silver salt, opens up new paths for the application of silver clusters in biological systems. 

Fig. 10 (a) Fluorescence image of methanol-fixed NIH3T3 cells loaded with peptide encapsulated silver clusters for 1 h at room temperature, (b) Time profile of the time series images of cell stained with silver nitrate showing the fast silver cluster emission centered in the nucleus at short times with a maximum at 320 nm. Note that black indicates an intermediate intensity level in this color scheme [57]... 

Silver nitrate, which is a caustic agent, is available as a stick or pen in combination with potassium nitrate and is suitable for the removal of warts on the hands and feet. It should be used with caution and patients are advised to protect the surrounding skin, as it can cause chemical burns. It can also cause staining of skin and fabric. 

Its action is similar to that of silver nitrate but it causes sharp pain and does not stain the dentine. 

A yellow to brown or black stain is produced,4 which is compared with a set of standard strips prepared under similar conditions. The chief difficulty encountered is to obtain a reliable and permanent set of standards especially is this the case with silver nitrate, the stains of which do not keep. The most satisfactory method5 of preparing such stains is to soak the filter paper in gum tragaeanth, dry it, soak it in silver nitrate solution, again dry it, expose to arsine under the requisite conditions and fix the stain by repeated soaking in very dilute ammonia and coating with paraffin. By the use of 66 per cent, silver nitrate solution, OT x 10-6 g, of As may be detected.6... 

After an electrophoresis run is complete, the gel must be analyzed to answer analytical or experimental questions. As most proteins are not directly visible, the gel must be processed to determine the location and amount of the separated proteins. The most common analytical procedure is staining. Proteins are usually stained with Coomassie brilliant blue or silver nitrate. 

Find the spots on the gel corresponding to landmarks on the film (see Note 26) and modify the size of the silver nitrate image adjusting it to the smaller one of film by the mean of adequate software. Actually the gel is larger than the film owing to silver staining procedure. 

Silver nitrate (AgNO ) Demo 4.1 Stains and bums skin. Irritates mucous membranes. Can cause blindness if splashed in eyes. Wear plastic gloves, goggles. 

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