Stab cultures

Loch, n. hole, opening cavity eye pore prison, -blende, /. perforated screen or diaphragm. -eisen, n. puncher, pimch. locben, v.t. perforate, punch, pierce, locherig, a. full of holes, perforated, porous. Locherstelle,/. open place, gap, hole. Loch-frass, m. pitting (as of metals), -karte,/. punch (ed) card, -kultur,/. (Bact.) stab culture. -scheibe, /. perforated disk, apertured disk. 

Stich-kultur, /. stab culture, -loch, n. tap hole, -pfropf, m. tap-hole plug, -probe, /. sample at random sample taken by tapping or piercing random test, spot check (Metal.) assay of tapped metal (Brewing) pricking teat. 

Preparation of Cultures. L. oenos ML 34 can be maintained as a stab culture (with transfer every four months) on a freshly prepared modified... 

Starter cultures of L. oenos ML 34 for inoculating wine are obtained by inoculating the grape juice medium with 1 vol % of a subculture (or another starter culture). The subculture is prepared by inoculating 5 ml of the grape juice medium from a stab culture. The cultures are incubated at room temperature until turbidity is seen,... 

For cultivation of fermentative species such as LAB, low-oxygen environments are relatively easily created by use of either stab cultures or agar... 

The ability to transfer microorganisms from one container to another without contamination is crucial to success in the microbiology laboratory. These techniques serve as the basis for subsequent work such as starter culture preparation or maintaining viable cultures in long-term storage. Transfer loops are normally used to transfer to the surface of agar (Petri plates and slants), whereas transfer needles are used to prepare stab cultures. Both implements are sterilized by heating in an open flame until red hot (Fig. 13.1). 

Select a colony from the Petri plate, slant, or stab culture. 

Maintenance of test organisms. The organisms used in microbiological assays, with few exceptions, are best stored in the freeze-dried state. For current use they are kept on nutrient agar slopes or, with the lactobacilli, as stab cultures, starting from a fresh freeze-dried tube occasionally. Keep such cultures at 4° and subculture them at weekly intervals. For use in the assay, prepare the inoculum as given in Tables 64 and 65. 

Stab culture Single strength basal medium + 1-5% agar and 2miug Vit. Bis per ml... 

Corti A, Solaro R, Chiellini E (2002) Biodegradation of poly(vinyl alcohol) in selected mixed microbial culture and relevant culture filtrate. Polym Degrad Stab 75 447-458... 

Tang B, Liaoa X et al (2010) Enhanced production of poly(vinyl alcohol)-degrading enzymes by mixed microbial culture using 1,4-butanediol and designed fermentation strategies. Polym Degrad Stab 95 557-563... 

Toothpicks obtainable from Boots Chemists (U.K.) but may be locally difficult to obtain. Ensure that they do not contain added bacteriocidal agents. (Sterilized pasteur pipettes are equally suitable as a way of stabbing out plaques from culture plates). 

To prepare plate cultures, frozen stocks are placed on dry ice and are stabbed with a hot wire loop. Material adhering to the loop is then plated onto the blood agar plates. Frozen stocks may require several passages to regain growth characteristics. 

Stock cultures of Lactobacillus casei are kept as stab inoculum in microassay culture agar. These are subcultured monthly in microassay culture agar. 

Lee W-H, Azizan MNM, Sudesh K (2004) Effects of culture conditions on the composition of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) synthesized by Comamonas acidovorans. Polym Degrad Stab 84 129-134... 

S.A.S. Alariqi, A. Pratheep Kumar, B.S.M. Rao, R.P. Singh, Biodegradation of y-sterilised biomedical polyolefins under composting and fungal culture environments. Polym. Degrad. Stab. 91, 1105-1116 (2006)... 

Lee HJ, Chd MH, Kim TU, Yoon SC (2001) Accumulation of polyhydroxyalkanoic acid containing large amounts of unsaturated monomers in Pseudomonas fluorescens BM07 utilizing saccharides and its inhibition by 2-bromooctanoic acid. Appl Environ Microbiol 67 4963-4974 Lee WH, Azizan MNM, Sudesh K (2004) Effects of culture conditions on the composition of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) synthesized by Comamonas acidovorans. Polym Degrad Stab 84 129-134... 

In the case of slants, place the loop at the bottom of the test tube and streak the culture toward the neck. With stabs, the needle is thrust into the agar and then withdrawn. 

Microorganisms are commonly stored on the appropriate solidified medium, either as slants or stabs (weeks) or for longer periods (months) as liquid cultures suspended in glycerol kept at low temperatures (—70°C/ —94°F). Alternatively, microorganisms can be preserved for very long term storage (years) by freeze-drying. Method 13.10.3 has been used to freeze-dry yeasts (C.M.L. Joseph, personal communication, 2005), and method 13.10.4 has been used successfully for lactic acid bacteria (Duke, 1979). 

Transfer cultures to the slants or stabs using the procedures outline in Section 13.8.1. 

Stock culture maintained on Liver tryptone agar stab Liver tryptone agar stab Malt agar slope Liver tryptone agar stab Yeast agar stab i... 

Nishida H, Konno M, Ikeda A, Tokiwa Y (2000a), Microbial degradation of poly(p-dioxanone) I. Isolation of degrading microorganisms and microbial decomposition in pure culture , Polym Degrad Stab, 68, 205-217. 

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