Spleen extraction

The most convincing evidence in favor of a uniform 3,5-diester linkage between nucleotides has been obtained by the action of various enzymes on synthetic diesters of known constitution.218 217 Ribonuclease and spleen extracts were found to act only on nucleoside 3-(benzyl hydrogen phosphates), but not on other isomers, to give nucleoside cyclic phosphates which are broken down further to give nucleoside 3-phosphates. It is concluded, by analogy, that polynucleotides, which are substrates for these enzymes, also possess ester groupings at the 3-positions, rather than at the... 

Since none of the compounds at the concentrations used showed a complete supression of splenomegaly, one would expect a residual viral activity in spleen extracts of mice, which received FLV suspensions preincubated with these compounds. Studies are now in progress to evaluate the leukemogenic activity of cell-free spleen extracts, prepared from mice inoculated with pretreated suspensions. Wu etal.63 have carried out such studies with RLV and rifamycin derivatives. They reported that the inoculation of mice with inocula from mice infected with RLV pretreated with AF-ABDP and AF/DNF1 did not cause splenomegaly. 

Figure 9.140 HPLC elution profile of a sample taken from a discontinuous assay of crude mouse spleen extract, using iV,-fluorenylmethoxycarbonyl-EEY(P)AA [Fmoc-EEY(P)AA] as substrate. Chromatography was performed on a C g Novapak column (10 cm X 8 mm) eluted isocratically with 36% acetonitrile-water (0.1% TFA) (v/v) at a flow rate of 2 mL/min. Peaks are due to (A) methanol and methanol-soluble compounds derived from the sample of crude homogenate, (B) Fmoc-EEY(P)AA (1238 pmol), and (C) Fmoc-EEYAA (195 pmol). Appropriate controls showed that no interfering compounds eluted in the position of the peptides. Inset Fluorescence monitoring of HPLC of Fmoc-EEYAA (75 fmol) eluted isocratically with 36% acetonitrile-water (0.1% TFA). Excitation and emission wavelengths, 268 and 307 nm, respectively, with gain X 100 and 10 mV chart scale. (From Nash et al., 1993.)...

Ashwell G, Hickman J. Formation of xylulose 5-phosphate from ribose 5-phsphate in spleen extracts. J. Am. Chem. Soc. 1954 76 5889. 

Polyerga therapy utilizes porcine spleen extract that is composed of various peptides, that is, of relatively short-chain proteins. It is noted in the reference that the sole U.S. distributor of Polyerga is European Lifestyle Products, POB 1345, Gibsonia, PA 15944, 724-934-3068. 

Nutrapon TLS-500. See TEA-lauryl sulfate Nutrapon W1367 Nutrapon WAC 3005 Nutrapon WAQ, Nutrapon WAQE 2364. See Sodium lauryl sulfate NutraSweet . See Aspartame Nutrex PG. See Glycosaminoglycans Nutrex PV. See Hydrolyzed com starch Nutrex RT. See Spleen extract Nutrex TM. See Thymus hydrolysate Nutricoi Konjac. See Konjac flour Nutrifos 088 Nutrifos BC Nutrifos Powd.. See Pentasodium triphosphate Nutrifos SK. See Trisodium dipotassium tri polyphosphate... 

Trideoxy-1,4-imino-5(4-methoxy phenyl)D-xylo-pentitol 3-acetate. See Anisomycin Tri-Derm SE. See Spleen extract Tri (P,P -dichloroisopropyl) phosphate CAS 13674-87-8... 

Sodium DNA Soluble proteoglycan Spinal cord extract Spleen extract Spleen hydrolysate... 

Thymus hydrolysate Trachea hydrolysate Tuna extract Udder extract Umbilical extract Yeast betaglucan Yeast polysaccharides Yogurt Yogurt filtrate Zinc citrate Zinc DNA Zinc yeast derivative biological additive, emulsions Spleen extract Thymus hydrolysate biological additive, hair care Hydrolyzed conchiorin protein Hydrolyzed fibronectin Hydroxypropyl chitosan Lysine lauroyl methionate biological additive, hand lotions Sodium beeswax... 

Spleen extract cell survival aid Sodium butyrate cellophane... 

Soybean (Glycine soja) extract Spinal cord extract . Spleen extract Spleen hydrolysate Stearoylglutamic acid... 

Zinc aspartate Zinc DNA Zinc yeast derivative conditioner, skin dandruff treatments Pyridoxine dicaprylate Pyridoxine dipalmitate conditioner, skin emulsions Spleen extract Thymus hydrolysate conditioner, skin eye care Hydrolyzed milk protein conditioner, skin hair care AMP-isostearoyl hydrolyzed soy protein AMP isostearoyl hydrolyzed wheat protein Behenamidopropyl dimethylamine lactate Benzyltrimonium hydrolyzed collagen Cocodimonium hydroxypropyl silk amino acids... 

Hydrogenated palm kernel oil 84540-05-6 Ovarian extract 84540-14-7 Nutrex RT Spleen extract Tri-Derm SE 84540-15-8 Stomach extract 84540-19-2 Testicular extract 84540-50-1... 

Hydrogenated palm kernel oil 283-094-8 Ovarian extract 283-105-6 Spleen extract 283-106-1 Stomach extract 283-110-3 Testicular extract 283-144-9... 

The quality of mRNA is important in generating recombinant antibodies. It is particularly important to ensure minimal ribosomal RNA contamination when cDNA is random primed for Ubraiy construction to reduce the proportion of clones which represent non-mRNA species. The spleen mRNA of immunized mice is presmnably derived mainly from plasma cells, as the level of Ig mRNA in these cells is up to 1000-fold greater than in resting B cells. For spleens, extraction of total RNA followed ly mRNA isolation on oligo(dT)-cellulose is recommended in order to effectively process the large number of cells present in a spleen. Cytoplasmic RNA Extraction Kit (see Protocol 2) followed by mRNA Purification Kit is hi y recommended. 

At least three lectin species have been detected in lymphocytes isolated from the thymus and spleen of BALB/c mice. Lectins specific towards jS-o-galactosides and 2-acetamido-2-deoxy-D-galactosides are found mainly in thymus extracts, and those specific towards a-D-mannosides in spleen extracts. 

The formation of Ru-5-P from Xu-5-P is catalyzed by another very active enzyme, phosphoketopentoepimerase (epimerase). This enzyme was first detected in spleen extracts by Ashwell and Hickman (16). It occurs in bacteria (341), muscle (97,324), and spinach (J. Hurwitz and B. L. Horecker, unpublished observation), although it has not been purified from the latter source. Highly purified preparations have been obtained from extracts of Lactobadlliis pentosus (186). At 25° the equilibrium mixture contains about 60% of Xu-5-P (186). 

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