Synovial fluid specimen

Measurements Normal load, cartilage deformation, friction cartilage wear and damage, biochemical analysis of cartilage specimens, synovial fluid, and wear debris sub-surface changes... 

Fluid specimens apply to abdominal washings, ascitic fluids, colonic washings, duodenal washings, gastric washings, pleural fluids, pericardial fluids, ovarian cyst fluids, synovial fluids, and sputa. 

MPO is an abundant azurophil granule product and ANCA auto-antigen which is found immunologically (16-29mg/ml synovial fluid) in all rheumatoid fluids [17]. Interestingly, only a minority of the synovial fluid specimens contained any MPO activity [17], Since MPO released from degranulating stimulated PMNs into the extracellular milieu is oxidatively inactivated... 

As discussed earlier in this section, OSHA has mandated that all U.S. laboratories have an exposure control plan. In addition, the National Institute for Occupational Safety and Health (NIOSH), a functional unit of the GDC, has prepared and widely distributed a document entitled Universal Pre-cautions that specifies how U.S. clinical laboratories handle infectious agents. In general it mandates that clinical laboratories treat aU human blood and other potentially infectious materials as if they were known to contain infectious agents, such as HBV, HIV, and other blood-borne pathogens. These requirements apply to all specimens of blood, serum, plasma, blood products, vaginal secretions, semen, cerebrospinal fluid, synovial fluid, and concentrated HBV or HIV viruses. In addition, any specimen of any type that contains visible traces of blood should be bandied using tliese Universal Precautions. 

A special note should be made concerning the doctoral thesis by Lawrence Malcom in 1976 [36]. This is an excellent study of cartilage friction and deformation, in which a device resembling a rotary plate rheometer was used to investigate the effects of static and dynamic loading on the frictional behavior of bovine cartilage. The contact geometry consisted of a circular cylindrical annulus in contact with a concave hemispherical section. It was found that dynamically loaded specimens in bovine synovial fluid yielded the... 

Two studies have suggested that the IR spectra of synovial fluid specimens provide the basis to diagnose arthritis and to differentiate among its variants.A NIR study demonstrated that osteoarthritis, rheumatoid arthritis, and spondyloarthropathy could be distinguished on the basis of the synovial fluid absorption patterns in the range 2000-2400 nm.< In that case, the pool of synovial fluid spectra was subject to principal component analysis, and eight principal component scores for each spectrum were employed as the basis for linear discriminant analysis (LDA). On that basis, the optimal LDA classifier matched 105 of the 109 spectra to the correct clinical designation (see Table 7). 

Although applications of this type are not analytical in the traditional sense, they may provide analytical information indirectly. The successful classification of IR spectra according to disease type implies that the composition of the specimen is altered in a characteristic fashion with the onset of disease - in this case the synovial fluid make-up reflects the presence and type of arthritis. This is an intriguing finding, particularly as it is very unlikely that the IR spectra are detecting any particular constituent that cannot be (and has not been) assayed... 

Blood and body fluids include bulk laboratory specimens of blood tissue, sanen, vaginal secretions, cerebrospinal fluid, synovial fluid, pleural fluid, peritoneal fluid, pericardial fluid, and amniotic fluid. Precautions do not apply to feces, nasal secretions, sputum, sweat, tears, urine, or vomit unless they contain visible blood. Handle free-flowing materials or items saturated to the point of dripping liquids containing visible blood or blood components. Pathological waste includes all discarded waste from renal dialysis contaminated with peritoneal fluid or blood visible to the human eye. Consider solid renal dialysis waste as medical waste if saturated and demonstrate the potential to drip/splash blood or other regulated body fluids. Waste sharps include any used or unused discarded article that may cause punctures or cuts. 

Blood and body fluids include bulk laboratory specimens of blood tissue, semen, vaginal secretions, cerebrospinal fluid, synovial fluid, pleural fluid, peritoneal fluid, pericardial fluid, and amniotic fluid. Precautions do not apply to feces, nasal secretions, sputum, sweat, tears, urine, or vomit unless... 

Many errors can occur during the collection, processing, and transport of biological specimens. Minimizing these errors win result in more reliable information for use by healthcare professionals. Examples of biological specimens that are analyzed in clinical laboratories include whole blood serum plasma urine feces saliva spinal, synovial, amniotic, pleural, pericardial, and ascitic fluids and various types of solid tissue. The National Committee for CMnical Laboratory Standards (NCCLS) has published several procedures for collecting many of these specimens under standardized conditio ns.In addition, the NCCLS has published documents related to sample collection and analysis for specialized tests, such as sweat chloride (see also Chapter 27). 

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