Specificity, oligosaccharides with

Removal of the oligosaccharide chain from the glycoprotein usually involves the use of one of three main methods treatment with NaOH-NaBH4, hydrazinolysis, or proteolysis.34-36 The NaOH-NaBH4 treatment is used to release, somewhat specifically, oligosaccharides O-linked to serine and threonine. Hydrazinolysis is used to break N-linkages, and proteolysis, to isolate glycopeptides. Each method apparently still has some drawbacks. 

In this paper, it has been shown that glucansucrases with different synthetic specificities can synthesize glucans with different structures and can synthesize oligosaccharides with different structures by acceptor reactions. The mechanisms by which these enzymes carry out the polymerization and by which the acceptor reactions occur has been discussed. The products resulting from the... 

Selectins are a family of plasma membrane lectins that mediate cell-cell recognition and adhesion in a wide range of cellular processes. One such process is the movement of immune cells (T lymphocytes) through the capillary wall, from blood to tissues, at sites of infection or inflammation (Fig. 7-33). At an infection site, P-selectin on the surface of capillary endothelial cells interacts with a specific oligosaccharide of the glycoproteins of circu-... 

Oligosaccharides with blood group activity are found in human urine (7) and in human milk (3). Materials with blood group activity are widely distributed in nature (1, 2, 3, 4, 5, 6, 7, 8, 9) as are various proteins, plant (19, 20), and animal hemagglutinins, (21, 22, 23) which show specificity for blood group determinants. 

The formation of oximes and hydrazones can be used to modify proteins due to some attractive properties, that is, some stability in aqueous media and compatibility with many functionalities of proteins [134], Their synthesis can be accomplished through the nucleophilic addition-elimination (condensation) of hydrazines and alkoxyamines with aldehydes and ketones, respectively. Typically, it has been used to modify the N-terminus [135-137] of peptides and proteins. For example, oxime formation has been used to modify BSA and diphtheria toxin with Shigella sonnei O-specific oligosaccharides [138],... 

Antibodies directed against recombinant erythropoietin have been obtained from rabbits immunized with the hormone and Freunds complete adjuvant [64], Two sets of antibodies are present in the serum of rabbits that have been immunized, Fig. (3C). The results of oxidation of the erythropoietin with periodate, Fig. (25A), reaction of the antibodies with lectins of known carbohydrate specificity, Fig. (25B) and inhibition of the antibodies with the structural monosaccharide residues of the hormone, Fig. (26), have established the types of antibodies to be anti-carbohydrate antibodies and the immunodeterminants for these antibodies are oligosaccharides with the following structure N-acetyl neuraminyl a(2->3)... 

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