Spatiotemporal control

Adaptor proteins are multi-domain proteins (Fig. 1) that interact with components of signaling pathways [1]. As a consequence of these interactions, adaptor proteins are able to regulate signaling events within the cell, providing spatiotemporal control and specificity, and influencing how a cell responds to a particular stimulus. 

Spatiotemporal Control of Nucleation and Growth in Enzymatic Supramolecular Polymerisation... 

Spatially confined self-assembly has been further confirmed by localizing ther-molysin on certain areas of a PEGylated surface. Upon immersion of this modified surface into a solution containing self-assembling precursors, nanostructures were formed in the vicinity of the enzyme, as observed through congo-red staining (Fig. 7c) [21]. Thus, enzyme-assisted self-assembly allows for construction of supramolecular polymers with spatiotemporal control, i.e. where and when they are required. 

Complementary observations of the aftereffects of enzymatic hydrolysis on the graft copolymers show spatiotemporally controlled degradation, leading to a new method for fine surface abrasion as well as for degradation-rate regulation of polymeric materials. 

Hatakeyama H, Kikuchi A, Yamato M et al (2007) Patterned biofunctional designs of ther-1172 moresponsive surfaces for spatiotemporally controlled cell adhesion, growth, and thermally 1173 induced detachment. Biomaterials 28(25) 3632-3643... 

The three methods taken together allow time-resolved spectroscopic characterization of propagating modes with far greater ease and completeness than has been possible in the past. They facilitate a number of important possibilities including spatiotemporal control over propagating modes, excitation and characterization of nonlinear lattice responses, tunable terahertz-frequency spectroscopy, and terahertz frequency and bandwidth signal processing. 

Prenylation has long been described as an obligate modification for membrane binding of the small GTPases so modified, as either structural mutation of the prenylated cysteines or pharmacological inhibition of the prenylation enzymes results in cytosolic proteins. However, prenylation is insufficient to support full biological activity. Because the diversity of small GTPase functionality requires very precise spatiotemporal control,... 

Site-specific and spatiotemporally controlled delivery of gene material. 

Hatakeyama H, Kikuchi A, Yamato M et al (2007) Patterned biofunctional designs of thermo-responsive surfaces for spatiotemporally controlled cell adhesion, growth, and thermally induced detachment. Biomaterials 28 3632-3643 Hem DL, Hubbell JA (1998) Incorporation of adhesion peptides into nonadhesive hydrogels useful for tissue resurfacing. J Biomed Mater Res 39 266-276 Huang J, Wang XL, Chen XZ et al (2003) Temperature-sensitive membranes prepared by the plasma-induced graft polymerization of N-isopropylacrylamide into porous polyethylene membranes. J Appl Polym Sci 89 3180-3187... 

Since L-CPTl deficiency is compatible with life, it is anticipated that a CPTIA knockout mouse model will be a viable model system. The value of such an animal model will increase if conditional (spatiotemporal) control of L-CPTl function can be achieved... 

X. Li, J. Wang, G. Su, Z. Zhou, J. Shi, L. Liu, M. Guan, Q. Zhang, Spatiotemporal control over growth factor delivery from collagen-based membrane, J. Biomed. Mater. Res. lOOA (2) (2012) 396-405. 

Hong L, Pan T (2010) Three-dimensional surface microfluidics enabled by spatiotemporal control of elastic fluidic interface. Lab Chip 10(23) 3271-3276... 

Figure 23.6 Study of ntia gene function in notochord development with hairpin cMOs. (a-e) Spatiotemporal control over ntia expression. Embryos were injected with hairpin ntia cMOs and irradiated at 6 or 12 hpf. (a) Nonirradiated embryos served as a control. (b) Embryos irradiated at 6hpf lacked the notochord, (c) Embryo irradiated at 12 hpf developed an abnormal notochord.

Lim RKV, Lin Q (2011) Photoinducible bioorthogonal chemistry a spatiotemporally controllable tool to visualize and perturb proteins in live cells. Acc Chem Res 44(9) 828—839. doi 10.1021/ ar200021p... 

The development of smart hydrogels as extracellular matrices has shown growing importance in cell culture [67]. By taking advantage of the spatiotemporal control of the photo-responsible compounds, Zhong and co-workers used the photo-triggered... 

Thiolene Thiol and unsaturated functional group (radical mediated) pH 6—8, reaction time <1 h Spatiotemporal control possible with select chemistries and using a photoinitiator Cell encapsulation, degradable three-dimensional cell culture ... 

Silva, E.A., Mooney, D.J., 2007. Spatiotemporal control of vascular endotheUal growth factor delivery fiom injectable hydrogels enhances angiogenesis. J. Thromh. Haemost 5 (3), 590-598. 

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