Single-stranded conformational

Progress in molecular biology has provided a new perspective. Techniques such as the polymerase chain reaction and single-strand conformation polymorphism analysis have greatly facilitated the molecular analysis of erythroenzymopathies. These studies have clarified the correlation between the functional and structural abnormalities of the variant enzymes. In general, the mutations that induce an alteration of substrate binding site and/or enzyme instability might result in markedly altered enzyme properties and severe clinical symptoms. 

CL Calabro, V., Mason, P. J., Filosa, S Civitelli, D., Cittadella, R.,Tagarclli, A., Martini, G., Bran-cati, C., and Luzzatto, L., Genetic heterogeneity of glucose-6-phosphate dehydrogenase deficiency revealed by single-strand conformation and sequence analysis. Am. J. Hum. Genet. 52, 527-536(1993). 

K11. Kanno, H Fujii, H., and Miwa, S Molecular heterogeneity of pyruvate kinase deficiency identified by single strand conformational polymorphism (SSCP) analysis. Blood 84 (Suppl. 1), 13a... 

Single-strand conformation polymorphism (SSCP) analysis... 

Bpgh, H.O., Zhu X.Q., Qian, B.-Z. and Gasser, R.B. (1999) Scanning for nucleotide variations in mitochondrial DNA fragments of Schistosoma japonicum by single-strand conformation polymorphism. Parasitology 118, 73-82. 

Gasser, R.B., Zhu, X.Q. and McManus, D.P. (1998c) Display of sequence variation in PCR-amplified mitochondrial DNA regions of Echinococcus by single-strand conformation polymorphism. Acta Tropica 71, 107-115. 

Kain, K.C., Craig, A.A. and Ohrt, C. (1996) Single-strand conformational polymorphism analysis differentiates Plasmodium falciparum treatment failures from re-infections. Molecular and Biochemical Parasitology 79, 167—175. 

Sheffield, V.C., Beck, J.S., Kwitek, A.E., Sandstrom, D.W. and Stone, E.M. (1993) The sensitivity of single strand conformation polymorphism analysis for the detection of single base substitutions. Genomics 16, 325-332. 

Stothard, J.R., Frame, IA. and Miles, MA. (1997) Use of polymerase chain reaction-based single strand conformational polymorphism and denaturing gradient gel electrophoresis methods for detection of sequence variation of ribosomal DNA of Trypanosoma cruzi. International Journal for Parasitology 27, 339-343. 

Teschauer, W., Mussack, T., Braun, A., Waldner, H. and Fink, E. (1996) Conditions for single strand conformation polymorphism (SSCP) analysis with broad... 

Gasser, R.B., Woods, W.G. and Bjorn, H. (1998) Distinguishing Oesophagostomum dentatum from Oesophagostomum quadrispinulatum developmental stages by a single-strand conformation polymorphism method. International Journal for Parasitology 28, 1903—1909. 

Beier DR, Dushkin H, Sussman DJ. Mapping genes in the mouse using single-strand conformation polymorphism analysis of recombinant inbred strains and interspecific crosses. Proc Natl Acad Sci USA 1992 89 9102-9106. 

Spire-Vayron de la Moureyre C, Debuysere H, Sabbagh N, Marez D, Vinner E, Chevalier ED et al. Detection of known and new mutations in the thiopurine S-methyltransferase gene by single-strand conformation polymorphism analysis. Hum Mutat 1998 12 177— 185. 

Single-strand conformation polymorphism (SSCP) Wastewater bioreactors (including denitrifying and phosphate-removal system, Chinese traditional medicine wastewater treatment system, beer wastewater treatment system, fermentative biohydrogen producing system, and sulfate-reduction system) Microbial community structures, diversity and distribution in different wastewater treatment processes, and relationship between the structures and the status of processes [157]... 

Snyder TM, McGown LB (2005) Multiplex single strand conformation polymorphism analysis by capillary electrophoresis with on-the-fly fluorescence lifetime detection. Appl Spectrosc 59 335-339... 

Jl. Jaksch, M., Gerbitz, K. D., and Kilger, C., Screening for mitochondrial DNA (Mt DMA) point mutations using non radioactive single strand conformation polymorphism (SSCP) analysis. Clin. Biochem. 28, 503-509 (1995). 

Maesawa, C Tamura, G Suzuki, Y., Ishida, K., Saito, K., and Satodate, R. (1992) Sensitive detection of p53 gene mutations in esophageal endoscopic biopsy specimens by cell sorting combined with polymerase chain reaction single-strand conformation polymorphism analysis. Jpn. J. Cancer Res. 83, 1253-1256. 

Saed G, Ladin D, Olson J (1998) Analysis of p53 gene mutations in keloids using polymerase chain reaction-based single-strand conformational polymorphism and DNA sequencing. Arch Dermatol 134 963-967... 

Jones AC, Austin J, Hansen N, Hoogendoorn B, Oefner PJ, Cheadle JP, O Donovan MC (1999) Optimal temperature selection for mutation detection by denaturing HPLC and comparison to single-stranded conformation polymorphism and heteroduplex analysis. Clin Chem 45 1133-1140... 

Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci USA 1989 86 2766-2770. 

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