Simultaneous fluorescence analysis

We prepared thin film Pt alloy electrodes by Ar-sputtering Pt and the second metal targets simultaneously onto a disk substrate at room temperature (thickness approximately 200 nm). The resulting alloy composition was determined by gravimetry and X-ray fluorescent analysis (EDX). Grazing incidence (i7= 1°) X-ray diffraction patterns of these alloys indicated the formation of a solid solution with a face-centered cubic (fee) crystal stmeture. 

Theory HPLC analysis of frusemide and its decomposition products is carried out by using simultaneous fluorescence and UV detection. 

The characterization of fresh and used automotive catalysts, which includes the examination of poisons accumulated in the catalysts, uses a variety of modem analytical techniques. The two principal tools, besides conventional chemical methods, are atomic absorption and, most important, X-ray fluorescence (XRF). The latter technique has been refined and adapted for the analysis of automotive catalysts to permit rapid and accurate determination of all constituents, including the inadvertent contaminants. An example of a simultaneous XRF analysis of... 

At present, there are advanced difference gel electrophoresis (DOGE) Systems and 2-D fluorescence difference gel electrophoresis (2-D DIGE) which enable the analyst to use simultaneously modern (more precise) methods of fluorescent analysis with 2-D electrophoresis (using internal patterns), aided by a fully integrated bioinformatics system. Such systems allow more complete differential protein analysis, while the application of internal standards eliminates differentiation between the intervals, thus ensuring that even the smallest differences will be detected irrespective of the multitude of components. This guarantees reproducibility of results and their statistical reliability. Such assays are one of the platforms employed in the research based on the proteomics method. 

In thTs communication, we will illustrate the application of the Video Fluorometer to simultaneous analysis of polycyclic aromatic hydrocarbons and warfarin and its metabolite 7-OH warfarin. We hope to convince the reader of the utility of "higher-order" strategies in fluorescence analysis made practicable by imaging detector based instrumentation. 

The data shown below illustrates how the concept of multidimensional analysis might be used to devise a simultaneous fluorescence assay of warfarin and its major metabolite, 7-0H warfarin. 

Finally, we point out the efficacy of higher order strategies in fluorescence analysis. We have shown that when the dimension of pH is added to the simultaneous analysis of warfarin and 7-OH warfarin an improvement in quantitation results. Other dimensions may be added as well, for example, a series of excitation emission matrices may be recorded as a function of chromatographic retention time, time after delta-function excitation, or as a function of concentration of an added quencher. New strategies will be required to analyze such large multi-dimension data sets, but the benefits will be even larger--the quantitative and qualitative analysis of ever more complex mixtures. 

X-Ray fluorescence is nondestructive and has significant advantages in simultaneous multielement analysis and ultramicroanalysis using electron beam excitation. It has found widespread industrial applications but as instrumentation is costly and complex in comparison with analytical atomic spectroscopy, the technique is not suitable for routine use in clinical chemistry. It seems unlikely that it can ever be more than a research tool. 

M12. Mitchell, D. G., and Johansson, A., Simultaneous multielement analysis using sequentially excited atomic fluorescence radiation. Specirochim. Acta, Part B 25, 175-182 (1970). 

The pulsed-source (time-resolved) method thus effects spectroscopic separation of the emission of several fluorescing species by making use of differences in their decay times rather than their fluorescence intensities. This is useful where strongly overlapping fluorescence spectra complicate simultaneous quantitative analysis without chemical or mechanical separation. 

H. Morgan, D. Holmes and N. G. Green, High speed simultaneous single particle impedance and fluorescence analysis on a chip, Curr. Appl. Phys., 6, 367-370 (2006). 

The total iron content may be determined directly in the soil by the X-ray fluorescence analysis or by a spectrographic method. In the case of classic methods, the soil sample should be first decomposed either by melting with Na2C03 or by the action of hydrofluoric acid. The melting procedure is more advantageous, since in this case further elements can also be determined simultaneously with iron. When it is only necessary to determine iron, it is advantageous to use the decomposition with hydrofluoric acid, which does not need the separation of silicic acid. In solution, iron is determined most frequently by the photometry, AAS or polarography. ... 

Dall Asta, C., Galaverna, G., Biancardi, A., Gasparini, M., Sforza, S., Dossena, A, Marchelli, R. (2004). Simultaneous liquid chromatography-fluorescence analysis of type A and type B trichothecenes as fluorescent derivatives via reaction with coumarin-3-carbonyl chloride. Journal of Chromatography A, VoL 1047, No. 2, (August 2004), 241-247, ISSN 0021-9673. 

A new photochrome-fluorescence-spin method for the simultaneous quantitative analysis of the redox status and viscosity of a medium has been developed [20], The method of the viscosity measurement is based on the use of double fluorescence-nitroxide molecules. 

X-ray fluorescence spectrometry is a technique for rapid simultaneous multielement analysis that exceeds the capabilities of normal clinical or toxicological laboratories. However, time-consuming sample preparation for trace elements and detection limits diminishes the usefulness to arsenic [157-159]. 

A spectrometer is an instrument that provides information about the intensity of radiation as a function of wavelength or frequency. The dispersing modules in some spectrometers are multichannel so that two or more frequencies can be viewed simultaneously. Such instruments are sometimes called polychromators. A spectrophotometer is a spectrometer equipped with one or more exit slits and photoelectric transducers that permit the determination of the ratio of the radiant power of two beams as a function of wavelength as in absorption spectroscopy. A spectrophotometer for fluorescence analysis is sometimes called a speciroftttorometer. 

Morgan, H., Holmes, D., Green, N.G., 2006. High speed simultaneous single particle impedance and fluorescence analysis on a chip. Curr. Appl. Phys. In AMN-2 (Second International Conference on Advanced Materials and Nanotechnology) Second International Conference on Advanced Materials and Nanotechnology vol. 6, pp. 367—370. http //dx.doi. org/10.1016/j. cap. 2005.11.020. 

Zybin A., Schurer-Patschan C. and Niemax K. (1992) Simultaneous multielement analysis in a commercial graphite furnace by diode laser induced fluorescence, Spectrochim. 

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