Silk nanofibres

Oroudjev, E., Soares, J., Arcdiacono, S., Thompson, J. B., Fossey, S. A., and Hansma, H. G. (2002). Segmented nanofibres of spider dragline silk Atomic force microscopy and single-molecule force spectroscopy. Proc. Natl. Acad. Sci. USA 99, 6460-6465. 

K. E. Park, S. Y. Jung, S. J. Lee, B-M Min, W. H. Park. 2006. Biomimetie nanofibrous scaffolds Preparation and characterization of ehitin/silk fibroin blend nanofibers. 

Kluge JA et al (2011) Versatile nanofibrous composites for soft tissue repair via silk protein incorporation. In Proceedings 2011 Orthopaedic Research Society annual meeting. Long Beach. ORS, Rosemont... 

Zhang K, Qian Y, Wang H et al (2010) Genipin-crosslinked silk fibroin/hydroxybutyl chitosan nanofibrous scaffolds for tissue-engineering application. J Biomed Mater Res 95 A 870-881... 

Sheng, X.Y., Fan, L.P., He, C.L., Zhang, K.H., Mo, X.M., Wang, H.S., 2013. Vitamin E-loaded silk fibroin nanofibrous mats fabricated by green process for skin care application. International Journal of Biological Macromolecules 56, 49—56. 

Park WH, Jeong L, Yoo DI, Hudson S (2004) Effect of chitosan on morphology and conformation of electrospun silk fibroin nanofibers. Polymers 45 7151-7157 Patnaik PR (2006) Dispersion optimization to enhance PHB production in fed-batch cultures of Ralstonia eutropha. Bioresour Technol 97 1994-2001 Patra SN, Bhattacharyya D, Ray S, Easteal AJ (2009) Electrospun poly(lactic add) based conducting nanofibrous networks. lOP Conference Series Mater Sci Eng doi 10.1088/1757-899X/4/l/012020 Peh KSH, Sodhi NS, de Jong J, Sekercioglu CH, Yap CAM, Lim SLH (2006) Conservation value of degraded habitats for forest birds in southern Peninsular Malaysia. Divers Distrib 12 572-581... 

Park, K.E., Jung, S.Y., Lee, S.J., Min, B.M., Park, W.H. Biomimetie nanofibrous seaffolds preparation and eharacterization of chitin/silk fibroin blend nanofibers. Int. J. Biolog. Macromol. 38, 165-173 (2006a)... 

Electrospun SF-based fibers were prepared from aqueous regenerated silkworm silk Bombyx mon)/PEO solutions to be used as scaffolds for tissue engineering (Jin et al. 2004). PEO supplied good mechanical properties to the electrospun fibers. An MeOH posttreatment induced an amorphous to silk p-sheet conformational transition. The electrospun silk membrane was washed with water to remove PEO in order to improve the cell adhesion and proliferation. These silk fibrous membranes were nonimmunogenic, biocompatible, and capable of supporting bone marrow stromal cell (BMSC) attachment. In another work, electrospun wool keratin/silk fibroin (WK/SF) blend nanofibers exhibited higher Cu + adsorption capacity than SF nanofibrous membrane (Ki et al. 2007). 

Ki, C.S., Gang, E.H., Um, I.C. and Park, Y.H. 2007. Nanofibrous membrane of wool keratose/ silk fibroin blend for heavy metal ion adsorption. 

Shanmugavel, S., Reddy, V. J., Ramakrishna, S., Lakshmi, B., Dev, V. G. (2014). Precipitation of hydroxyapatite on electrospun polycarpolactone/aloe vera/silk fibroin nanofibrous scaffolds for bone tissue engineering. Journal of Biomaterials Applications, 29(11), 46-58. 

The incorporation of nanohydroxyapatite into a chitosan/silk fibroin nanofibrous membrane scaffold may provide a favorable mi-... 

Kaplan et al. [28], combined bone morphogenetic proteins and HAp nanoparticles in the mixtures for electrospinning composite silk nanofibers. They have shown that this combination resulted in the highest calcium deposition in nanofibrous electrospun scaffolds for mesenchymal stem cell cultures. 

Figure 2.12 shows the morphology of cells after 3, 7 and 14 days on spider silk nanofibers. Figure 2.13 shows the morphology on spider silk nanofibers with 1% CNT. On day 3 the cells were attached to the nanofibrous scaffolds... 

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