Serum Silver nitrat

Serum and bile thiocyanates are raised. See also HYDROCYANIC ACID. Unstable and easily oxidized. Explosive polymerization may occur on storage with silver nitrate. Potentially explosive reactions with benzyltrimethylammonium hydroxide + pyrrole, tetrahydrocarbazole + benzyltrimethylammonium hydroxide. Violent reactions with strong acids (e.g., nitric or sulfuric), strong bases, azoisobutyronitrile, dibenzoyl peroxide, di-tert-butylperoxide, or bromine. 

Serum Solvent extraction cleanup on 10% silver nitrate on silica gel column GC/ECD No data 93.7 at 41 pg/L Needham et al. 1980 ... 

The effects of inorganic salts on plasma cholinesterase (E16) are largely contradictory. Fruentova (F9) reported that divalent cations are more effective inhibitors of horse serum cholinesterase than are monovalent ions, whereas divalent ions are frequently reported to have a marked activating effect (H38, T8, VI). Lithium and sodium nitrates have been shown by in vitro studies of the reaction of human plasma cholinesterase with benzoylcholine to have identical inhibition profiles (W21), while sodium and potassium chlorides had very similar inhibitory actions on the hydrolysis of acetylcholine by human plasma (H47). Silver nitrate, copper sulfate, and mercuric chloride are powerful inhibitors of F. polycolor butyrylcholinesterase (N2). Cohen and Oosterbaum (C12) concluded that activation by cations occurring at the usual substrate concentration is highly dependent on the experimental conditions. This supposition is very relevant to the somewhat random choice of buffers and substrates in the work reported above. 

Figure 14. 2-D PAGE analysis of P40. One hundred and fifty rg of P40 (batche 3) were loaded onto a 5-8 IPG strip by in-gel rehydration. After focusing, proteins were separated in the second dimension on a 15% homogeneous gel before (A) transfer to a PVDF membrane and immunoblotting with the rabbit anti-HCP serum, or (B) silver nitrate staining.

Preparation from [i- C] Linoleic Acid. (9Z,llf )-[l-i" C]-Octadecadienoic acid was prepared by incubating [l-i" C]-linoleic acid with an enzyme, Unoleate isomerase, obtained from the rumen bacterium Butyrivibrio fibrisolvens (11). This isomerization was carried out with a mixture of 0.2 mL of the enzyme preparation, [l-i" C]-linoleic acid (0.2 pCi 0.12 mg) and 4 mg of bovine serum albumin in 1 mL of 0.1 moI/L potassium phosphate buffer (pH 7). After separation of fatty acids from the reaction mixture and formation of methyl esters with diazomethane, methyl (9Z,ll )-[l-i C]-octadecadienoate was purified by preparative silver nitrate thin-layer chromatography. 

Fig. USB. Two-dimensional fractionation of human serum lipids on a layer partly impregnated with silver nitrate [182]. Adsorbents silica gel G and silica gel G-silver nitrate (5%) solvents 1 direction petrol ether (BP 40—60° C)-diethyl ether-acetic acid (85 + 15 + 1), 2 direction petrol ether (BP 40—60° C)-diethyl ether-acetic acid (75 + 25 + 1) visualisation charring by heating with chromic acid/ sulphuric acid amount 300 fxg 26 Thin-Layer Chromatography, 2nd Edition...

Almost 200 pesticides were analyzed in various systems using dichloromethane and ethyl acetate (129). These pesticides were visualized by the following selective detection methods (a) o-tolidine-potassium iodide, (b) p-nitro-benzene-diazonium-fluoborate, (c) silver nitrate with UV irradiation, (d) p-dimethylaminobenzaldehyde, (e) bioassays with either fungispores of Aspergillus nigeror an enzyme inhibition method. When horse blood serum was the enzyme source, acetylcholine iodide was its substrate in the presence of 2,6-dichloro-phenol-indophenol. Naphthyl acetate was applied as the substrate for the human blood plasma esterases. The Rf values and detection limits were also published (129). 

---