Serum profiling

Parissis JT, Adamopoulos S, Venetsanou KF, Mentzikof DG, Karas SM, Kremastinos DT. Serum profiles of C-C chemokines in acute myocardial infarction possible implication in postinfarction left ventricular remodeling. J Interferon Cytokine Res 2002 22(2) 223-229. 

A. M. Haag, J. Chaiban, K. H. Johnston, and R. B. Cole. Monitoring of Immune Response by Blood Serum Profiling Using Matrix-Assisted Laser Desorption/Ionization Time-of-FlightMass Spectrometry. J. Mass Spectrom., 36(2001) 15-20. 

Pharmacokinetics The absorption rate of Gonal-F following subcutaneous or intramuscular administration was found to be slower than the elimination rate. Hence the pharmacokinetics of Gonal-F are absorption rate-limited. After intravenous administration to pituitary down-regulated, healthy female volunteers, the serum profile of FSH appears to be described by a two-compartment open model with a distribution half-life of about 2 to 2.5 hours. Steady-state serum levels were reached after 4 to 5 days of daily administration. 

Organic acid analysis could be performed in other biological specimens [14, 22] when targeting specific analyte(s) in previously diagnosed patients, or fetuses at risk for a specific condition. Plasma/serum profiling is not helpful in the laboratory evaluation of patients without a specific diagnosis, and should be avoided [2]. 

Merry BJ, Holehan AM. Serum profiles of LH, FSH, testosterone and 5 alpha-DHT from 21 to 1000 days of age in ad libitum fed and dietary restricted rats. Exp Gerontol 1981 16 431-444. 

B9. Berney, T., Gasche, Y., Robert, J., Jenny, A., Mensi, N., Grau, G., Vermeulen, B., and Morel, P., Serum profiles of interleukin-6, interleukin-8, and interleukin-10 in patients with severe and mild... 

GutjahRjC., Murphy, D., Lurking, A., Koenig, A., Janitz, M., O Brien, J., Korn, B., Horn, S., Lehragh, H., Cahill, D. J. (2005). Mouse protein arrays from a THl cell cDNA library for antibody screening and serum profiling. Genomics 85, 285-296. 

A nonredundant human protein chip for antibody screening and serum profiling. 

HaitwickeJ al. (HIO) studied the use of high-ionic-strength solutions for the precipitation of serum proteins. In this technique a saturated solution of ammonium sulfate was added to serum and the supernatant was analyzed by RPLC after centrifugation. The recoveries for serum nucleosides and xanthine alkaloids were good, ranging from 93 to 100%. In addition, the serum profile showed no interference from this extraction technique. 

The use of the enzyme purine nucleoside phosphorjiase for enzymatic peak identification is illustrated by the RPLC serum profile from a patient with severe depression. Based upon the retention time, the peak eluting at approximately 15 min was tentatively identified as inosine (Fig. 14A). Cochromatography with an inosine reference compound resulted in a subsequent increase in peak area for the compound of interest (Fig. 14B). Fur-fiiermore, stopped-flow UV spectra indicated a similarity between the inosine standard and the peak tentatively identified as inosine (Fig. 15). 

Fig. 13 Mean serum profiles of 3-keto-desogestrel and ethinylestradiol after a 21 day continuous intravaginal administration of two prototype combination contraceptive vaginal rings (CVR). The profiles from an oral combination tablet (Marvelon) is plotted for comparison. (From Ref. l)...

In 2009, Ladd et al. studied diagnostic BMs in cancer by nanoproteomics approaches based on a combination of antibody microarrays and a SPRi detection system. This work also showed the potential of SPRi as a suitable methodology for studying serum profiles in biomarker discovery projects (32). 

Blatter M, Fluckiger U, Entenza J, et al. Simulated human serum profiles of one daily dose of ceftriaxone plus netilmicin in treatment of experimental sheptococcal endocardihs. Antimicrob Agents Chemother 1993 37 1971-1976. 

Fic. 6, Serum profiles of SM-C/IGF-I (lines) and growth hormone (shaded) in 3 rats sampled at 15 minute intervals over 6 hours. The mean peak value ( SD) for 10 animals was 1.21 0.29 U/ml, and the mean trough value was 0.60 0.20 U/ml, with a mean peak interval of 1.93 0.47 hours. [From Baxter et al. (B19) with permission.]... 

Ueshiba, H. Segawa, M. Hayashi, T. Miyachi, Y Irie, M. Serum profiles of steroid hormones in patients with Cushing s syndrome determined by a new HPLC/RIA method. Clin.Chem., 1991, 37, 1329— 1333... 

Gutjahr C, Murphy D, Lueking A, et al. (2005). Mouse protein arrays from a THl cell cDNA library for antibody screening and serum profiling. Genomics. 85 285-296. 

IMAC3, Metal binding (most widely used for serum profiling) 100 mM CUSO4 humid chamber 15 min, neutralize 5 min with 0.1 M NaOAc pH 4.0 0.1 M sodium phosphate buffer (pH 7.0), 0.5 M NaCl, 10 mM imidazol, pH 7.0 (see Note 2) Three times, 5 min with binding buffer, once with water Can also be loaded with nickel or gallium (no equilibration needed) (23,24)... 

Teeguarden JG, Calafat AM, Ye X, Doerge DR, Churchweh Ml, Gunawan R, Graham MK. Twenty-four hour human urine and serum profiles of bisphenol a during high-dietary exposure. Toxicol Sci 2011 123 (l) 48-57. 

Decreased Apo A1 appears to be a major component of the dyslipi-demic serum profile in patients with atherosclerotic occlusive disease of the lower extremities (461). Despite similar TC and HDL-C levels, A1 HDL is more anti-atherogenic than A1/A2 HDL in transgenic mice (462). This is because lecithinxholesterol acyltransferase (LCAT) and cholesteryl ester trasfer protein (CETP) are present mainly in Lp A1 and because Lp A1 A2 can inhibit Lp A1 -promoted cholesterol efflux. Also, mice overexpressing Apo A1 have more cholesterol efflux and are more protected against atherosclerosis mediated through decreased foam cell... 

Want, E. J. O MaiUe, G. Smith, C. A. Brandon, T. R. Uritboonthai, W Qin, C. et al. Solvent-dependent metabolite distribution, clustering, and protein extraction for serum profiling with mass spectrometry. Anal. Chem. 2006, 3, 743-752. 

Qu, Y., Adam, B.-L., Yasui, Y, Ward, M.D., Cazares, L.H., Schellhammer, P.F., Feng, Z., Semmes, O.J. and Wright, G.L., Boosted decision tree analysis of surface-enhanced laser desorption/ionization mass spectral serum profiles discriminates prostate cancer from noncancer patients. Clin. Chem., 4S, 1835-1843 (2002). 

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