Humid chamber

Sodium valproate is hygroscopic. The rate of moisture absorption was tested in a humidity chamber using a Cahn Electro Balance and the results are shown in Table V (5). 

Fig. 2. Diagram of humidity chamber made from a plastic box. Water at the bottom of the chamber ensures a high humidity once the lid covers the chamber, this prevents significant evaporation of the slides placed on shelves. The sections are encircled by the lipid content from a DAKO pen, limiting the amount of antibody used. The chamber is wrapped in tinfoil from the start when used for alkaline phosphatase experiments. 

Add 20% swine serum in TBS for 20 min in a humidity chamber (see Fig. 2) sealed with tinfoil. Pour off the swine serum and dry with paper towels around the circles made by the DAKO pen. 

Incubate with secondary antibody 1 20 (Anti-Rabbit Immunoglobulins coupled with alkaline phosphatase, DAKO code no. D0306) in swine serum/TBS for 30 min. in a humidity chamber. 

Develop with (naphthol AS-BI phosphate/Fast Red, fresh made) for 20 min in the dark (in tinfoil-sealed humidity chambers). For preparation of developer, see Subheading 5.1.1.1, item 4. 

Incubate grids in a humid chamber on large drops of a saturated aqueous solution of sodium metaperiodate (prepared overnight, 1 g in 5 ml Aqua dest) for 30 60 min at room temperature. 

The microdilution method was employed for antibacterial and antifungal activity tests. Media were placed into each well of the 96 well-microplate. Extract solutions at 1,024 pg/ml were added into first raw of microplates and twofold dilutions of the compounds (512-0.25 pg/ml) were made by dispensing the solutions to the remaining wells. Ten microliters of culture suspensions were inoculated into all the wells. The sealed microplates were incubated at 35°C for 24 and 48 h in humid chamber. The lowest concentration of the extracts that completely inhibit macroscopic growth was determined and minimum inhibitory concentrations (MICs) were... 

Remove coverslip and blot excess buffer from sample, taking care not to directly touch sample. Add enough TdT labeling reaction buffer to cover sample. Cover with plastic coverslip and incubate in humid chamber for at least 60 min at 37°C. Stop labeling reaction by removing coverslip and washing sample in 2X SSC for 10 min at room temperature. 

Humid chamber leveling tray with lid, for horizontal antibody application. 

Humidity chamber (Shandon Lipshaw, Pittsburgh, PA or to make your own, see Note 1). 

For optimizing pretreatment conditions, treat a series of tissues (sectioned from the same tissue block) with different concentrations of proteinase K (5, 10, 15, 20, 25, 30 pg/mL) and incubate for 15 min at 37°C in the humidity chamber. 

In a clean, RNase-free humidity chamber, add some sterile DEPC-treated water or wet some paper towels and place them on the bottom of the humidity chamber. Place the humidity chamber in a 37°C water bath or in an incubator to bring the temperatnre to eqnilibrinm. 

Remove the humidity chamber from the incubator, drain off the enzyme by tilting the slide on a paper towel. Inactivate the enzyme activity by putting the slides into a staining dish with 0.1 M glycine solution for 20 min. 

Incubate the slides overnight (12-16 h) at room temperature (25-28°C) in the humidity chamber. 

Carefully wipe the area around each tissue section, place the slides flat in the humidity chamber, and check the horizontal level of the chamber. 

Drying of the antidigoxigenin antibody. (Always check the surface level of the humidity chamber before the addition of antidigoxigenin antibody solution.)... 

Hydration. The treated aluminum specimens were placed In a Blue M Humidity chamber maintained at 65 C and 95% relative humidity for specified time periods, removed and then dried. 

Wedge Test. The adhesive bond durabilities of the Inhibitor-treated 7075-T6 surfaces were evaluated by wedge tests (ASTM D-3762) on bonded specimens using the FM 123-2 epoxy adhesive to simulate the epoxy primer. The specimens were placed In a humidity chamber at 65°C and 95% relative humidity and removed at specified time Intervals to record the crack tip locations after each examination, they were returned to the humidity chamber. 

Remove the slide and apply an antibody for one of the haptens onto the slide and incubate for approximately 15 min in a humidity chamber/box (see Note 20) at room temperature. If the first hapten to visualize is DNP, anti-DNP antibody is applied. For a direct detection with the use of an enzyme-conjugated anti-hapten antibody, skip steps 2 and 3. 

Apply a freshly prepared chromogen reagent mixture to the slide and incubate them in a humidity chamber/box at room temperature. Observe the color development under the light microscope and terminate the reaction by soaking in rinse solution when it is appropriate (see Note 22). 

Tissue sections should not be dried during immunological BISH detection. A humidity chamber/box can be assembled by placing wet paper towels in a tray and covering it with a plastic wrap sheet. PAP pen is often used for creating a hydrophobic barrier that is dissolved with xylene for immunohistochemistry. However, because BISH detection... 

Place the slides horizontally in a humid chamber, e.g., Petri dishes containing wet filter paper, and store in a refrigerator at least for 24 h, better for 48-72 h. 

Cut the antiserum slots Ah after finishing the electrophoresis and remove the agar within the slots. Pour antiserum into the slot(s) and store the slide in a humid chamber at 37 °C for 4-6 h or at 4 °C for 24-48 h. Visualize the precipitation lines as described in Protocol 4.8.4. 

Dissolve the antigen (pure protein or hapten-carrier conjugate) with a concentration between 0.1 and 50 pg/ml in Soln. A. Pipet 0.1-0.2 ml of the antigen solution into the wells of a 96-well microtiter plate (high binding capacity) and incubate on a shaker at RT for 1 h or at 4 °C in a humid chamber overnight. 

Moisture uptake was not observed when brinzolamide was stored uncapped for 4 weeks in a 40°C / 75% relative humidity chamber. Thus the compound is essentially non-hygroscopic. 

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