Schizosaccharomyces pombe cell cycle

The CaMK Cascade and Cell Cycle in Schizosaccharomyces Pombe... 

For application with triple-quadrapole and especially (J-LIT instruments, a selected-reaction monitoring (SRM) procedure was developed for the sensitive and selective detection of phosphopeptides in proteomes with known amino-acid sequences [26]. A list of SRM transitions of potential phosphopeptides is generated for all expected tryptic peptides in the mixtme with Ser, Thr, or Tyr and for double-and triple-charge ions in the mass range of m/z 400-1600. The number of transitions included is limited by the maximum cycle time of 10 s, which assures that peptides in a 30 s wide peak are at least analysed twice. The procedure was applied to the cell cycle regulatory protein Cyclin B from Schizosaccharomyces pombe. 

No events have been described which uniquely characterize the G1 phase. The G1 phase appears to perform two functions the temporary or permanent arrest of cell division in eukaryotes is normally achieved by cells maintaining themselves in the G1 phase of the cell cycle (Prescott, 1970) also the terminal part of the G1 must contain events concerned with the initiation of DNA synthesis. Certain organisms, for example, the fission yeast Schizosaccharomyces pombe (Bostock et al., 1966), the multinucleate slime mold Physarum polycephalum (Guttes et al., 1967), and Amoeba proteus (Ord, 1968), do not exhibit a G1 phase. A number of mammaUan cells appear to lack a G1... 

Most work in yeast genetics has been performed with two species, Sac-char omyces cerevisiae and Schizosaccharomyces pombe. For a detailed description of the respective life cycles, see Mortimer and Manney in Volume 1 of this series. It must be pointed out that both organisms can be cultivated as haploids and, in the case of Sacch. cerevisiae, in stable diploid forms as well. Since there are a great variety of yeasts of the Saccharomyces type which do not behave in this ideal way, all genetic experiments should be performed with strains currently used by yeast geneticists. Such strains are physiologically dioecious (see Esser 2) for definition of this term), whereas many strains isolated from nature are self-compatible and haploid cells fuse uncontrollably to form diploids. Typically, Saccharomyces strains... 

Chen BR, Runge KW (2009) A new Schizosaccharomyces pombe chronological lifespan assay reveals that caloric restriction promotes efficient cell cycle exit and extends longevity. Exp Gerontol 44 493-502... 

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