Reverse chemical genomics

The resultant master ORF clones should be checked by several different criteria DNA sequence, protein productivity, and the structure of produced proteins see Note 5). Once these master ORF clones whose quality has been well-checked become available to the research community, a wealth of time, labor, and cost will surely be saved and provide a solid basis for reverse chemical genomic approaches. In this context, many groups working on ORFeome cloning have made their ORF clones open to the research community For human ORF clones, the ORFeome collaboration is actively working to generate a complete set of human ORF clones as an international collaboration (http // www.orfeomecollaboration.org/) Schizosaccharomyces pombe ORF clones are already available at the DNA bank of RIKEN... 

Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study... 

Key words Reverse chemical genomics, G-protein coupled receptor, Eukaryote genome, Gene... 

Application of SEVENS and GRIFFIN to Reverse Chemical Genomics... 

Figure 4 shows the flow of GPCR gene analysis for the purpose of supporting the reverse chemical genomics approach. 

Fig. 4. Work flow of GPCR gene classification and in-silico functional prediction using SEVENS and GRIFFIN, to support reverse chemical genomics.

The main objective in gene module analysis of reverse chemical genomics is to find new functional relationships between different chemicals. Once a set of gene modules annotated by GO and KEGG is obtained, check the common function names for every combination of query chemicals with a significant p value threshold. 

Figure 2.3 Diagram representing forward and reverse chemical genomic paradigmsP2]...

Neckers L. Development of small molecule Hsp90 inhibitors utilizing both forward and reverse chemical genomics for drug identification. Curr Med Chem 2003 May 10(9) 733-739. 

Case studies for reverse chemical genomics applications... 

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