Salmonella lyphimurium

Chloroacetaldehyde has been reported to be an inhibitor of DNA synthesis and to form DNA adducts it is mutagenic in Salmonella lyphimurium and in Chinese hamster cellsA Limited in vivo genotoxicity studies with chloroacetaldehyde were negative. ... 

Dimethyl hydrogen phosphite was not mutagenic to several strains of Salmonella lyphimurium, but it did cause sister chromatid exchanges and chromosomal aberrations in the Chinese hamster CHO line. ... 

The DNTs appear to cause mutations in Salmonella lyphimurium assays after metabolic activation. In vivo 2,4-DNT causes unscheduled DNA synthesis in rat hepatocytes and chromosomal aberrations in human lympho-qn es both 2,4- and 2,6-DNT have induced DNA adducts in rat liver. ... 

Diphenylamine was not mutagenic in the Ames Salmonella lyphimurium test. ... 

Propene has been reported to be nonmu-tagenic to both Escherichia coli and Salmonella lyphimurium, either with or without metabolic activation interestingly, the purported reactive metabolite of propene, propene oxide, is widely accepted as a mutagen. Furthermore, propene oxide forms hemoglobin adducts in exposed animals. ... 

In chronic 2-year feeding studies of the mixed isomer, there was no evidence of carcinogenicity in rats given up to 300 ppm or mice given up to 250ppm in the diet." Tricresyl phosphate was not mutagenic in Salmonella lyphimurium, nor did it induce chromosomal aberrations or sister chromatid exchange in Chinese hamster ovary cells."... 

A study of the mutagenicity of ZDC used a battery of in vitro mutagenicity studies. ZDMC and ZDEC were positive in both the Ames Salmonella lyphimurium assay and the human lymphoqnie cell mutation assay but not in the mouse lymphoma cell mutation assay. In contrast, ZDBC was not positive in the assays. 

Schmid E, Googelmann W, Bauchinger M. 1986. Formaldehyde-induced cytotoxic, genotoxic and mutagenic response in human lymphocytes and Salmonella lyphimurium. Mutagenesis 1 427-431. 

Salmonella lyphimurium, 582 esters, nickcl(II) complexes hydrolysis, 424... 

Two in vitro mutagenidty studies were conducted on Glarinol G-80 a reverse mutation assay in five histidine-requiring strains of Salmonella lyphimurium (an Ames test) and an in vitro cytogenetics assay in human lymphocytes (chromosome aberration test) (5). Both studies were conducted in accordance with Good Laboratory Practice (GLP) and in accordanc e with OEGD guidehne 471 and 473 respectively. 

The primary sequence of the 0-subunit (397 residues) is also known, and extensive studies have been reported on the 3-dimensional structure of the 0202 multienzyme complex, in particular for the enzyme from Salmonella lyphimurium. The enzymes from Ecoli and S. lyphimurium are very similar the respective a-subunits both consist 268 amino acid residues and differ at 40 positions (15%), while the 0-monomers display only 3.5% difference in primary sequence (both consist of 397 residues, and only 14 of these are different). Crystallographic studies show that the active sites of the a- and 0-subunits are 25 A apart and connected by a tunnel, which presumably serves to carry the one metabolic intermediate (indole) from the active site of the a-subunit to the active site of the p-subunit. The kinetics of this substrate channeling have been studied by chemical quench-flow and stopped-flow methods- (I.P. Crawford J.Ito Proc. NatL Acad. ScL USA. 51 (1964) 390-397 B.P.Nichols CYanofeky Proc NatL Acad. Sci. USA. 76 (1979) 5244-5248 S-A. Ahmed et al. J. Biol. Chan. 260 (1985) 3716-3718 CCHyde et al. J. Biol. Chem. 263 (1988) 17857-17871 K.S. Anderson et al. J. Biol. Chem. 266 (1991) 8020-8033]... 

SM has been investigated for its ability to produee point mutations in baete-ria in the absence of an exogenous metabolie aetivation system. Salmonella lyphimurium strains TA98 and TAIOO were used as well as the original G46 strain. A clear positive result was obtained in G46 and also TAIOO. ... 

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