Saccharomyces cerevisiae heterologous

Saccharomyces cerevisiae is well characterized biochemically and genetically and was the organism of choice for most of the eady experiments. However, heterologous expression seems to be better in some of the industrial strains of yeasts such as Pichiapastoris Hansenulapolymorpha Kluyveromyces lactis and Yarrowia lipolytica (25—28). 

Cira LA et al (2008) Heterologous expression of Fusarium oxysporum tomatinase in Saccharomyces cerevisiae increases its resistance to saponins and improves ethanol production during the fermentation of Agave tequilana Weber var. azul and Agave salmiana must. Antonie Van Leeuwenhoek. Int J Gen Mol Microbiol 93 259... 

There are two yeast expression hosts that have an established track record for high-level production of heterologous proteins, namely Saccharomyces cerevisiae and Pichia pastoris. HTP expression screening using microplate formats has been reported for both these yeasts by Lang and coworkers (Holz et ah, 2002, 2003 Boettner et ah, 2002). In both cases standard protocols have been miniaturized with cells cultured in either 1.5 ml cultures in 96-deep-well plates for S. cerevisiae or 2 ml cultures in 24-deep-well plates for P. pastoris. Soluble... 

Hemrika, W., R. Renirie, S. Macedo-Ribeiro, A. Messershmidt, and R. Wever. 1999. Heterologous expression of the vanadium-containing chloroperoxidase from Curvu-laria inaequalis in Saccharomyces cerevisiae and site-directed mutagenesis of the active site residues His(496), Lys(353), Arg(360), and Arg(490). J. Biol. Chem. 274 23820-23827. 

Beaudoin, F., Gable, K., Sayanova, O., Dunn, T., Napier, J. A. A Saccharomyces cerevisiae gene required for heterologous fatty add elongase activity encodes a microsomal beta-keto-reductase. J. Biol. Chem. 2002, 277 11481-11488. 

Robinson AS, Bockhaus JA, Voegler AC et al (1996) Reduction of BiP levels decreases heterologous protein secretion in Saccharomyces cerevisiae. J Biol Chem 271 10017-10022... 

Cai, D. Y., and Klinman, J. P., 1994, Copper amine oxidase heterologous expression, purification and characterisation of an active enzyme in Saccharomyces cerevisiae. Biochemistry 33 7647n7653. 

Heterologous expression studies of Saccharomyces cerevisiae reveal two distinct trypanosomatid CaaX protease activities and identify their potential targets. Eukaryot Cell 8 1891-1900. 

Napier, J.A., Hey, S.J., Lacey, D.J. and Shewry, P.R. Identification of a Caenorhabditis elegans delta6-fatty-acid-desaturase by heterologous expression in Saccharomyces cerevisiae. Biochem J, 330 (Pt 2) (1998) 611-614. 

Hie expression of recombinant mammalian liver ferrochelatases in Escherichia coli [4, 5], allowed the production of increased amounts of the enzyme and led to the unexpected discovery of a [2Fe-2S] cluster in the mammalian enzymes (mouse [6] and human [7]). The large amoimts of protein made available using heterologous over-producing systems allowed detailed spectroscopic studies on the cluster-containing ferrochelatases. Yeast Saccharomyces cerevisiae) ferrochelatase, which is devoid of an iron-sulfur cluster, has also been extensively studied and several site-directed mutants have been produced [8], making it a powerful model system to study the mechanism of this enzyme. 

Qiu X, Hong H, MacKenzie SL. (2001a) Identification of a delta 4 fatty acid desaturase from Thraustochytrium sp. involved in the biosynthesis of docosahexanoic acid by heterologous expression in Saccharomyces cerevisiae and Brassica juncea. J Biol Chem 276 31561-31566. 

Laoteng, K., Mannontarat, R., Tanticharoen, M., and Cheevadhanarak, S., A6-Desaturase gene of Mucor rouxii with high similarity to plant A6-desaturase and its heterologous expression in Saccharomyces cerevisiae, Biochem. Biophys. Res. Commun. 2000, 279, 17-22. 

Sleep D, Belfield G P, Ballance D J, Steven J, Jones S, Evans L R, Moir P D, Goodey A R (1991). Saccharomyces cerevisiae strains that overexpress heterologous proteins. Bio/technol. 9 183-187. 

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