Kluyveromyces lactis

Saccharomyces cerevisiae is well characterized biochemically and genetically and was the organism of choice for most of the eady experiments. However, heterologous expression seems to be better in some of the industrial strains of yeasts such as Pichiapastoris Hansenulapolymorpha Kluyveromyces lactis and Yarrowia lipolytica (25—28). 

Figure 4.4 The production of whey protein and Kluyveromyces lactis from whey.

Morlino, G.B., Tizzani, L., Fleer, R. et al. (1999) Inducible amplification of gene copy number and heterologous protein production in the yeast Kluyveromyces lactis. Applied and Environmental Microbiology, 65 (11), 4808 1813. 

Although not well understood, demethiolation has been noted in some fungi.38 Moreover, evidence for a required enzyme activity was obtained for lactococci (used in cheddar cheese production)40 and a relatively high level of demethiolase activity was present in Kluyveromyces lactis, a cheese-ripening yeast (see below).41... 

In addition to bacterial conversion of L-methionine to cheese aroma compounds, certain cheese-ripening yeasts have been implicated. They include De-baromyces hansenii, Geotrichum candidum, and Yarrowia lipolytica, in addition to Kluyveromyces lactis and Saccharomyces cerevisiae (previously noted). Of these yeasts, Geotrichum candidum was most effective at producing sulfur compounds with the major product being S-methyl thioacetate, with smaller amounts of MT, DMS, DMDS, and DMTS. Kluyveromyces lactis had a similar profile, but produced a much smaller amount of S-methyl thioacetate than did G. candidum. S-Methyl thioacetate is formed by a reaction of MT and acetyl-CoA (Equation 7) ... 

Work that may prove of immense industrial significance is the isolation from Kluyveromyces lactis, by Dickson and Markin,394 of the gene for /3-D-galactosidase by use of recombinant-DNA techniques. The DNA from the yeast was partly digested with endonuclease, and the product was joined to a plasmid DNA by means of DNA ligase. The ligated DNA was then successfully used to transform a lactose-nega-... 

Rose et al., 1989) as well as in the plant Nicotiana tabacum (Denecke et al., 1991), and DDEL in Kluyveromyces lactis (Lewis and Pelham, 1990). The dnaK proteins have a 10- to 20-residue G,A,Q-rich segment, which leads into a highly charged, predominantly acidic termination. In this case, no specialized function has been ascribed to the carboxy-terminal sequence motif. 

Kluyveromyces lactis overexpressing KlPDIUl and KlEROl, Appl. Environ. 

LACTASE ENZYME PREPARATIONS from KLUYVEROMYCES LACTIS... 

Kegel A, Martinez P, Carter SD, Astrom SU. Genome wide distribution of illegitimate recombination events in Kluyveromyces lactis. Nucleic Acids Res. 2006 34 1633-1645. 

Galactosidases of Aspergillus oryzae, Penicillium multicolor, Kluyveromyces lactis, and Escherichia coli were reported to form, 1-6-linkages [21]. Employment of /3-galactosidase of Xantomonas manihotis and recombinant Bacillus circulans (bga C gene) also gave considerable amounts of, 1-6-linked disaccharides [30],... 

Based on results of De Deken.3016 A form of Kluyveromyces lactis. 

Only just over 10% of yeast species utilize lactose (see Table III), and most of these species also utilize D-galactose. /3-D-Galactosidase (EC 3.2.1.23) activities have been studied chiefly for two species, namely, Kluyveromyces /ragtits221,223,313,577-587 and Kluyveromyces lactis.545,588... 

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