Saccharomyces cerevisiae gene mutation assay test

Brusick and Matheson (1976) reported that 1,1-dimethylhydrazine failed to increase reversions in Salmonella typhimurium or Saccharomyces cerevisiae gene mutation assays with or without metabolic activation. A concentration-related response was observed in the mouse lymphoma assay (with activation). Dominant lethal tests were negative. 

Positive results from the Saccharomyces cerevisiae gene mutation assay indicate that a substance induces point mutations by base pair substitution or frameshift in the genome. Base pair substimtion and frameshift is explained under the in vivo test. 

OECD, 1986b, Test Guideline 480, Genetic Toxicology Saccharomyces cerevisiae, Gene Mutation Assay. 

The mutagenic potential of diisopropyl methylphosphonate was investigated using the Ames assay. The compound was obtained from two different sources and tested on Salmonella typhimurium strains TA-1535, TA-1537, TA-1538, TA-98, and TA-100, both with and without S-9 activation. The compound did not demonstrate mutagenic activity in any of the assays (Hart 1980). Diisopropyl methylphosphonate was also negative for gene mutation in Saccharomyces cerevisiae (Hart 1980). 

Mehta, R.D. von Borstel, R.C. (1985) Tests for genetic activity in the yeast Saccharomyces cerevisiae using strains D7-144, XV185-14c and RM52. Prog. Mutat. Res., 5, 271-284 Myhr, B., Bowers, L. Caspary, W.J. (1985) Assays for the induction of gene mutations at the... 

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