Reversed-phase high performance liquid chromatograph

McKern, N. M., Edskes, H. K., and Shukla, D. D., Purificatiion of hydrophilic and hydrophobic peptide fragments on a single reversed phase high performance liquid chromatographic system, Biomed. Chromatogr., 7, 15, 1993. 

Dachtler, M., Kohler, K., and Albert, K. 1998. Reversed-phase high-performance liquid chromatographic identification of lutein and zeaxanthin stereoisomers in bovine retina using a C30 bonded phase. J. 

Chloupek, R.C., Hancock, W.S., Marchylo, B.A., Kirkland, J.J., Boyes, B.E., Snyder, L.R. (1994). Temperature as a variable in reversed-phase high-performance liquid chromatographic separations of peptide and protein samples, n. Selectivity effects observed in the separation of several peptide and protein mixtures. J. Chromatogr. A 686, 45-59. 

Pan et al. [82] used a reversed phase high performance liquid chromatographic method for the determination of miconazole nitrate and its related substances. 

The silanophilic character of 16 reversed-phase high-performance liquid chromatographic columns was evaluated with dimethyl diphenycyclam, a cyclic tetraza macrocycle [101]. The method is rapid, does not require the removal of packing material, and uses a water-miscible solvent. The results demonstrate two points first, cyclic tetraza macrocycles offer substantial benefits over currently used silanophilic agents second, the method can easily differentiate the performance of various columns in terms of their relative hydrophobic and silanophilic contributions to absolute retention. 

T Kinoshita, Y Kasahara, N Nimura. Reversed-phase high-performance liquid chromatographic resolution of non-esterified enantiomeric amino acids by derivatization with 2,3,4,6-tetra-O-acetyl-P-D-glucopyranosyl isothiocyanate and 2,3,4 tri-O-acetyl-a-D-arabinopyranosyl isothiocyanate. J Chromatog 210, 77, 1981. 

GRADIENT ELUTION FOR THE REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC SEPARATION OF COLOUR PIGMENTS IN THE EXTRACTS OF PAPRIKA (<CAPSICUM ANNUUM). ELUENT A, METHANOL-ACETONITRILE (80 20, V/V) ELUENT B, BIDISTILLED WATER... 

F. I. Kanaze, C. Gabrieli, E. Kokkalou, M. Georgarakis and I. Niopas, Simultaneous reversed-phase high-performance liquid chromatographic method for the determination of diosmin, herperidin, and naringin in different citrus fruit juices and pharmaceutical formulations. J. Pharm. Biomed. Anal. 33 (2003) 243-249. 

Court, W. A., Hendel, J. G., and Elmi, J. (1996a). Reversed-phase high-performance liquid chromatographic determination of ginsenosides of Panax quinquefolium. ]. Chromatogr. A 755, 11-17. 

Kuronen P, Volin P, Laitalainen T. 1998. Reversed-phase high-performance liquid chromatographic screening method for serum steroids using retention index and diode-array detection. J Chromatogr B 718(2) 211-224. 

Price, W.P., Jr., Edens, R., Hendrix, D.L., and Deming, S.N. (1979), Optimized Reverse-Phase High-Performance Liquid Chromatographic Separation of Cinnamic Acids and Related Compounds, Anal. Biochem., 93, 233-237. 

Teltes J, Levsen K. 1989. Reversed phase high-performance liquid chromatographic determination with photodiode-array detection of nitroaromatics from former ammunition plants in surface waters. J High Resolut Chromatogr 12 613-619. 

Friedman, M., Levin, C. E. (1992). Reversed-phase high-performance liquid chromatographic separation of potato glycoalkaloids and hydrolysis products on acidic columns. J. Agric. Food Chem., 40, 2157-2163. 

A reversed-phase high performance liquid chromatographic method for the determination of salvinorin A, a psychotropic diterpene isolated from the Mexican sage Salvia divinorum, has been developed. Extracts from several plant collections were examined on a C-18 column with UV detection and isocratic elution with acetonitrile water (45 55). This assay allowed quantification of salvinorin A in extracts of leaves and stems of A , divinorum and has also been applied to the screening of related species for the production of salvinorin A. Levels of salvinorin A in leaves range from 0.89 to 3.70 mg/g dry weight. 1999 John Wiley Sons, Ltd. 

Many scales, either empirical or measured, have been proposed for the hydrophobicity of amino acid residues in proteins (Nakai and Li-Chan, 1988). The most extensive study on tlje hydrophobicity index of amino acids was published by Wilce et al. (1995). The authors derived four new scales of coefficients from the reversed-phase high-performance liquid chromatographic retention data of 1738 peptides and compared them with 12 previously published scales. 

Richheimer, S.L., Kent, M.C.. and Bemart, M.W. 1994. Reversed-phase high-performance liquid chromatographic method using a pen-tafluorophenyl bonded phase for analysis of tocopherols. J. Chromatogr A 677 75-80. 

MR Euerby. Effect of differing thiols on the reversed-phase high-performance liquid chromatographic behaviour of o-phthalaldehyde-thiol-amino acids. J Chromatogr 454 398-405, 1988. 

JL Glajch, JJ Kirkland, J Kohler. Effect of column degradation on the reversed-phase high-performance liquid-chromatographic separation of peptides and proteins. J Chromatogr 384 81-90, 1987. 

MG Scanlon, PKW Ng, DE Lawless, W Bushuk. Suitability of reversed-phase high-performance liquid chromatographic separation of wheat proteins for long-term statistical assessment of breadmaking quality. Cereal Chem 67 395-399, 1990. 

BA Marchylo, DW Hatcher, JE Kruger, JJ Kirkland. Reversed-phase high-performance liquid chromatographic analysis of wheat proteins using a new, highly stable column. Cereal Chem 69 371-378, 1992. 

A paired-ion, reversed-phase high-performance liquid chromatographic method was developed for the simultaneous determination of sweeteners (dulcin, saccharin-Na, and acesulfame-K), preservatives (sodium dehydroacetate, SA, salicyclic acid, BA, succinic acid, methyl-para-hydroxybenzoic acid, ethyl-para-hydroxybenzoic acid, n-propyl-para-hydroxybenzoic acid, n-butyl-para-hydroxybenzoic acid, and isobutyl-para-hydroxybenzoic acid), and antioxidants (3-tertiary-butyl-4-hydroxyanisole and tertiary-butyl-hydroquinone). A mobile phase of acetonitrile-50 ml aqueous tr-hydroxyisobutyric acid solution (pH 4.5) (2.2 3.4 or 2.4 3.6, v/v) containing 2.5 mM hexadecyltrimethylammonium bromide and a Clg column with a flow rate of 1.0 ml/min and detection at 233 nm were used. This method was found to be very reproducible detection limits ranged from 0.15 to 3.00 p,g. The retention factor (k) of each additive could be affected by the concentrations of hexadecyltrimethylammonium bromide and a-hydroxyisobu-tyric acid and the pH and ratio of mobile phase. The presence of additives in dried roast beef and sugared fruit was determined. The method is suitable for routine analysis of additives in food samples (81). 

S. M. Gaines and J. L. Bada, Reversed-phase high-performance liquid chromatographic separation of aspartame diastereomeric decomposition products, J. Chromatogr., 389 219 (1987). 

Mopper [41] has discussed developments in the reversed phase high performance liquid chromatographic determination of amino acids in estuarine and sea water. He describes the development of a simple, highly sensitive procedure based on the conversion of dissolved free amino acids to highly fluorescent, moderately hydrophobic isoindoles by a derivatisation reaction with excess o-phthaldehyde and a thiol, directly in... 

In this reversed phase high performance liquid chromatographic method for neutral and cationic metal chelates with azo dyes, tetraalkylammonium salts are added to an aqueous organic mobile phase. The tetraalkylammonium in salts are dynamically coated on the reversed stationary support. As a result of the addition of tetraalkylammonium salts, the retention of the chelates is remarkably reduced. Tetrabutylammonium bromide permits rapid separation and sensitive spectrophotometric detection of the vanadium(V) chelate with 2-(8-quinolylazo)-5-(dimethylamino)-phenol, making it possible to determine trace vanadium(V). 

Mantoura and Llewelyn [3] developed a reversed phase high performance liquid chromatographic system for a rapid (about 20min) separation and quantification of 14... 

Wood etal. (1988) developed the reversed-phase high-performance liquid chromatographic (HPLC) method for piperine determination in black pepper and its oleoresins. ft employs bonded C18 stationary phase (ODS-2) and acetonitrile-aqueous acetic acid mobile phase with UV detection. As the spectropho-tometric method which invariably yields higher results because of the contributions from other alkaloids such as piperyline and piperettine, the HPLC method relates more to piperine. Utilizing the UV absorption property of piperine, spectrophotometric estimation methods were developed by different groups using solvents such as benzene, ethanol, ethylene dichloride, acetone, ethyl acetate, chloroform and cyclohexane. Sowbhagya et al. (1990) proved that extractability with acetone was very efficient. 

Wood, A.B., Barrow, M.L. and James, D.J. (1 988) Piperine determination in pepper (Piper nigrum L.) and its oleoresins a reversed phase high performance liquid chromatographic method. Flavour and Fragrance journal 3(2), 55-64. 

Gana et al. developed and validated a reversed-phase high performance liquid chromatographic method for the kinetic investigation of the chemical and enzymatic hydrolysis of benazepril hydrochloride [37]. Kinetic studies on the acidic hydrolysis of benazepril hydrochloride were carried out in 0.1 M hydrochloric acid solution at 50, 53, 58 and 63°C. Benazepril hydrochloride appeared stable in pH 7.4 phosphate buffer at 37°C, and showed susceptibility to in vitro enzymatic hydrolysis with porcine liver esterase (PLE) in a pH 7.4 buffered solution at 37°C. 

Najera, A.I., Perea, S., Barcina, Y., de Renobales, M. and Barron, LJ.R. (1999) A reverse-phase high performance liquid chromatographic method for analyzing complex mixtures of triglycerides. Application to the fat fraction of an aged cheese. J. Am. Oil Chem. Soc., 76, 399-407. 

Hoffman, N.E. and Liao, J.C. Reversed phase high performance liquid chromatographic separations of nucleotides in the presence of solvophobic ions. Anal. Chem, 1977, 49, 2231-2234. 

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