Response to Inoculation

Antigen Injected (mg) Average Intrinsic Association Constants for Binding of c-DNP-Lysine to Anti-DNP Antibodies  

Increases in cross-reactivity with time are also due to a combination of the above factors. First, an antibody with high affinity for one determinant would be expected to have a lower but still measurable affinity for structurally similar determinants located on other proteins. Therefore the high affinity antibodies produced later exhibit broader specificities. Second, as a greater variety of antibodies appear in response to the multiple determinants of an antigen the probability of similarity between a given antibody type and a structurally related determinant of some other antigen increases. 

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Fester, T., W. Maier et al. (1999). Accumulation of secondary compounds in barley and wheat roots in response to inoculation with an arbuscular mycorrhizal fungus and co-inoculation with rhizosphere bacteria. Mycorrhiza 8(5) 241-246. 

Vierheilig, H., H. Gagnon et al. (2000). Accumulation of cyclohexenone derivatives in barley, wheat and maize roots in response to inoculation with different arbuscular mycorrhizal fungi. Mycorrhiza 9(5) 291-293. 

Thorpe, J. R., and Hall, J. L., 1984, Chronology and elicitation of changes in peroxidase and phenylalanine ammonia-lyase activities in wounded wheat leaves in response to inoculation with Botrytis cinerea, Physiol. Plant Pathol. 25 363-379. 

Kuc, J., Henze, R.E., Ullstrup, A.J., Quackenbush, F.W. Chlorogenic and caffeic acids as fungistatic agents produced by potatoes in response to inoculation with Helminthospcrium carbonum. J Amer Chem Socl956 78 3123. 

The problem of developing an immunoassay is basically one of isolating a monoclonal antibody with the required reactivity and specificity. These antibodies are produced by an animal s immune system in response to inoculation with an antigen. In the work reported here, the animals used were mice (Balb/c) or rabbits (New Zealand White). The antigen was, of course, different for the development of each antibody. 

Gordon et al.,71 have reported the isolation of capsidiol (143) an antifungal compound which is produced by the fruit of sweet peepers in response to inoculation with fungi Recently Bailey et al.,72 have isolated capsidiol (143) from Nicotiana tabacum and Nicotiana clevelandii following infection with tobacco necrosis virus. (TNV) It has been assigned the following structure... 

Growth and nitrogen fixation by subterranean clover in response to inoculation, molybdenum application and soil amendment with lime. Soil Biol. Biochem. 17 791-6. 

Jimsonweed - Datura stramonium. Like other Solanaceae, Datura stramonium produces antifungal sesqulterpenold compounds In response to inoculation with Monlllnla fructlcola and several other fungi (Ref. 39). Three of the four phytoalexins thus far identified are also accumulated by other Solanaceous species. Lubimin (2) is also produced both In potato and eggplant, capsldlol (20) is the main phytoalexin in sweet pepper fruit, and hydroxylubimln (5) is also found In potato. The fourth compound 2,3-dihydroxygermacrene, is unique to Datura and could serve as an almost direct precursor of lubimin and 4-hydroxylubimin as well as other Solanaceae phytoalexins (Ref. 39a). 

Jones, A.M., Thomas, V., Bennett, M.H., Mansfield, J., and Grant, M. 2006. Modifications to the Arabidopsis defense proteome occur prior to significant transcriptional change in response to inoculation with Pseudomonas syringae. Plant Physiol 142 1603-1620. 

The PAP method was pioneered by Sternberger in 1979 (1). The method uses an immunological sandwich amplification and the enzyme peroxidase to effect a signal. The unique feature of this procedure is the enzyme/antibody solution, the PAP immune complex. The horseradish peroxidase enzyme, itself an immunogenic protein, is used to inoculate a given species, and a polyclonal immune response is generated against the enzyme. This antiserum is harvested and placed in solution with the enzyme so that immune complexes form that... 

Miethling R et al (2000) Variation of microbial rhizosphere communities in response to crop species, soil origin, and inoculation with Sinorhizobium meliloti L33. Microb Ecol 40 43 Gray ston S J et al (2001) Accounting for variabUity in soU microbial communities of temperate upland grassland ecosystems. SoU Biol Biochem 33 533... 

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