Renal transplantation cell culture

Harmon, W.E., Parkman, R., Gavin, P.T., Grupe, W.E., Ingelfunger, J.R., Yunis, E.J. and Levey, R.H. (1982). Comparison of cell-mediated lympholysis and mixed lymphocyte culture in the immunologic evaluation for renal transplantation. J. Immunol. 129 1573-1577. 

McCauley J, Studer R, Craven P, Murray S. The effects of cyclosporine A, cyclosporine G, and FK 506 upon prostaglandin production in renal mesangial cells in culture. Transplant Proc 1991 23 3141-3142. 

Chan P, Chapman JR, Morris PJ. Glycosuria an index of cyclosporine nephrotoxicity. Transplant Proc 1987 19 1780. Nakahama FI. Stimulatory effect of cyclosporine A on endothelin secretion by a cultured renal epithelial cell line, LLC-PK1 cells. Eur J Pharmacol 1990 180 191-192. 

Other mediators have also been related to CsA-in-duced functional nephrotoxicity. Increased plasma level of adenosine due to reduced uptake by red blood cells was observed in CsA-treated renal transplant recipients [183]. In the same way, rats receiving CsA showed increased concentration of adenosine in renal artery paralleled by a decrease in mRNA expression for Aj and A2a renal adenosine receptors [184]. Experimental use of selective Aj adenosine receptors antagonists or theophylline resulted in contradictory results with some authors finding renal hemodynamic and functional protection whereas other did not [38, 185-187]. Moderate increases in plasma vasopressin were observed in CsA-treated renal transplant recipients [188] and CsA enhanced vasopressin-induced rise in intracellular calcium in cultured glomerular mesangial... 

There are several lines of evidence demonshahng that CSA causes endothelial cell injury. CSA has a direct cytotoxic effect on cultured endothelial cells and inhibits human umbilical endothelial cell proliferahon [74,75]. CSA increases the plasma level of endothelial damage markers, such as Von Willebrand factor, endothelin tissue factor pathway inhibitor, P-selectin and thrombin-antithrombin complexes in renal and heart transplant patients [76-79]. Similarly, the release of Von... 

Potier M, Wolf A, Cambar J. Comparative study of cyclosporin A, cyclosporin G, and the novel cyclosporin derivative iMM 125 in isolated glomeruli and cultured rat mesangial cells a morphometric analysis. Nephrol Dial Transplant 1998 13 1406-1411. Klein IH, Abrahams A, van EdeT, Hene RJ, Koomans HA, Ligtenberg G. Different effects of tacrolimus and cyclosporine on renal hemodynamics and blood pressure in healthy subjects.Transplantation 2002 73 732-736. 

Ghiggeri GM, Altieri P, Oleggini R, Valenti F, Ginevri F, Perfumo F, Gusmano R. Cyclosporine enhances the synthesis of selected extracellular matrix proteins by renal cells"in culture". Different cell responses and phenotype characterization.Transplantation 1994 57 1382-1388. 

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