Reiterative recombination

Wingler LM, Cornish VW (2011) Reiterative recombination for the in vivo assembly of libtaries of multigene pathways. Proc Natl Acad Sci USA 108 15135-15140... 

A potentially general method of identifying a probe is, first, to purify a protein of interest by chromatography (qv) or electrophoresis. Then a partial amino acid sequence of the protein is deterrnined chemically (see Amino acids). The amino acid sequence is used to predict likely short DNA sequences which direct the synthesis of the protein sequence. Because the genetic code uses redundant codons to direct the synthesis of some amino acids, the predicted probe is unlikely to be unique. The least redundant sequence of 25—30 nucleotides is synthesized chemically as a mixture. The mixed probe is used to screen the Hbrary and the identified clones further screened, either with another probe reverse-translated from the known amino acid sequence or by directly sequencing the clones. Whereas not all recombinant clones encode the protein of interest, reiterative screening allows identification of the correct DNA recombinant. 

Osborn BL, Olsen HS, NardeUi B, Murray JH, Zhou JX, Garcia A, Moody G, Zaritskaya LS, Sung C (2002) Pharmacokinetic and pharmacodynamic studies of a human serum albumin-interferon-alpha fusion protein in cynomolgus monkeys. J Pharmacol Exp Ther 303 540-548 Ozes ON, Reiter Z, Klein S, Blatt LM, Taylor MW (1992) A comparison of interferon-Conl with natural recombinant interferons-alpha antiviral, antiproliferative, and natural kiUer-inducing activities. J Interferon Res 12 55-59... 

Reiter, Y., and Pastan, I. (1998). Recombinant Fv immunotoxins and Fv fragments as novel agents for cancer therapy and diagnosis. Trends Biotechnol., 16, 513-520. 

Burgess K, Liaw AI, Wang N, Combinatorial technologies involving reiterative division/coupling/recombination statistical considerations, J. Med. Chem., 37 2985-2987, 1994. 

Reiter Y. Recombinant immunotoxins in targeted cancer cell therapy. Adv Cancer Res 2001 81 93-124. 

The mark of the French tradition is unmistakable in Rouelle s lectures, albeit modified by Boerhaave s and Stahl s theories. True to the longstanding didactic tradition at the Jardin, Rouelle began with a short theoretical section in which he dealt with principles and instruments. He reiterated, for example, the analytic-synthetic ideal by defining chemistry as a physical art which by means of certain operations and instruments teaches us to separate from bodies several substances which enter into their composition and to recombine them anew among them or with... 

Cohen CJ, Saiig O, Yamano Y, Tomaru U, Jacobson S, Reiter Y (2003) Direct phenotypic analysis of human MHC class I and-gen presentadon Visualizadon, quandtadon, and in situ detecdon of human vual epitopes using pepdde-specific, MHC-restiicted human recombinant andbodies. J Immimol 170 4349 361. 

Reiter LT, Murakami T, Koeuth T, Pentao L, Muzny DM, Gibbs RA, et al. A recombination hotspot responsible for 2 inherited peripheral neuropathies is located near a mariner transposon-Uke element. Nature Genet 1996 12 288-97. 

Denkberg, G. Lev, A. Eisenbach, L. Benhar, I. Reiter, Y., Selective targeting of melanoma and apes using a recombinant antibody with tcr-like specificity directed toward a melanoma differentiation antigen,./. Immunol. 2003, 171, 2197-2207... 

As mentioned above, both electrons and holes must be efficiently separated and both must reach the particle surface. Even when both reach the surface, recombination may still predominate if both are not consumed in appropriate fast reactions. It is worth reiterating that the requirements for fast reactions are twofold. The first is favorable energetics. The redox couples must be included within the band gap as shown in FIGURE 2. But, it is also necessary to maximize the rate of interfacial electron transfer.- This is essentially a standard problem of electrochemical kinetics and, as such, one for which there are extensive precedents. Above, we mentioned the general theoretical rules. Here, we will give more attention to specific examples. In most cases, experimental results can provide precedents. 

Reiter, R. S., Feldman, K. A., Williams, J. G. K., Rafalski, J. A., Tingey, S. V., and Scolnik, P A. (1992) Genetic linkage of the arabidopsis genome, methods for mapping with recombinant inbreds and random amplified polymorphic DNAs. Proc. Natl Acad. Sci. USA 89, 1477-1481. 

Reiter Y, Pastan I. Antibody engineering of recombinant Fv immunotoxins for improved targeting of cancer Disulfide-stabilized Fv immunotoxins. Clin Cancer Res 1996 2 245-252. 

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