Recombination site

Especially with LTG GaAs, materials became available that were nearly ideal for time-resolved THz spectroscopy. Due to the low growth temperature and the slight As excess incorporated, clusters are fonned which act as recombination sites for the excited carriers, leading to lifetimes of <250 fs [45], With such recombination lifetunes, THz radiators such as dipole anteimae or log-periodic spirals placed onto optoelectronic substrates and pumped with ultrafast lasers can be used to generate sub-picosecond pulses with optical bandwidths of 2-4 THz. Moreover, coherent sub-picosecond detection is possible, which enables both... 

Others impurities such as carbon present in the sample may play a major role in the reduction of the photocatalytic efficiency so far as they behave as recombination sites of electron-hole pairs [309],... 

Golden, J. W., Mulligan, M. E. and Haselkorn, R. (1987) Different recombination site specificity of two developmentally regulated genome rearrangements. Nature, 327, 526-9. 

The BP reaction is a recombination reaction that transfers a DNA fragment (such as a PCR product) flanked by attB recombination sites into an E. coli vector, called Donor vector containing attV sites, to produce a new plasmid, called Entry clone. This reaction is catalyzed by the BP Clonase mix of recombination proteins. In this reaction, the ccdE gene is removed from the Donor vector, thereby allowing the transformants to grow. 

PCR amplification with specific primers containing recombination sites ... 

Fig. 1. Outline of the strategy to construct GST-fused expression plasmids by the in vitro recombination-assisted method. Am, Gm, and Cm are abbreviations for ampicillin-, gentamicin-, and chloramphenicol-resistance, respectively. The figure also indicates the ccdB gene encoding a toxin targeting the co//essential DNA gyrase and the phage X recombination sites (attB, attP, attL, and attR).

FIGURE 4.3 Schematic representation comparing the full and deconstructed virus vector strategies, (a) TMV expression vector, (b) Provector system for rapid assembly of viral amplicons in planta. P promoter, TMV Pol TMV polymerase, MP movement protein, GOI gene of interest, CP capsid protein, T terminator, RS recombination site. (Adapted from Gleba et al. (2004). Curr. Opin. Plant Biol., 7, 182-188.)... 

In principle, interfacial recombination processes can be inhibited by modifying the interface. The use of t-butylpyridine in the DSSC electrolyte solution to increase its photovoltage is one example [2,97]. We wished to explore general methods for passivating interfacial recombination sites in DSSCs that might allow the use of a variety of redox couples and therefore facilitate making a viable solid-state DSSC. 

These reactions would produce a net e /h recombination and, conse-quendy, net null cycles, decreasing the rate of degradation [37]. As for salicylic acid cited earlier, the formation of surface-adsorbed cation radicals was invoked to increase the charge-carrier recombination rate and thus lower the quantum yields for degradation [27]. Added redox reagents can also act as recombination sites, so that the overall photocatalytic rate could increase, decrease, or remain unchanged [40]. 

Homologous genetic recombination can involve any two homologous sequences. The second general type of recombination, site-specific recombination, is a very different type of process recombination is limited to spe-... 

To complete the reaction, the process must be repeated at a second point within each of the two recombination sites (steps (3) and (4)). In some systems, both strands of each recombination site are cut concurrently and rejoined to new partners without the Holliday intermediate. The exchange is always reciprocal and precise, regenerating the recombination sites when the reaction is complete. We can view a recombinase as a site-specific endonuclease and ligase in one package. 

The sequences of the recombination sites recognized by site-specific recombinases are partially asymmetric (nonpalindromic), and the two recombining sites align in the same orientation during the recombinase reaction. The outcome depends on the location and orientation of the recombination sites (Fig. 25-39). If the two sites are on the same DNA molecule, the reaction either inverts or deletes the intervening DNA, determined by whether the recombination sites have the opposite or the same... 

FIGURE 25-39 Effects of site-specific recombination. The outcome of site-specific recombination depends on the location and orientation of the recombination sites (red and green) in a double-stranded DNA molecule. Orientation here (shown by arrowheads) refers to the order of nucleotides in the recombination site, not the 5 —>3 direction. 

System Recombinase/ recombination site Type of recombination Function... 

For a simple system such as 2-propanol, which decomposes to acetone, with no appreciable chain reactions, the QY reaches a plateau at very low light intensity.74) The precise value depends sensitively upon the characteristics of the particular film, probably depending upon the presence of trace impurities and defects, which could act as recombination sites. For a system in which there are radical chain reactions, such as acetaldehyde, QY can continue to increase, even at very low lnorm values, and can in principle exceed unity.75)... 

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