Site-specific recombination genome

Hartley, J. L., Temple, G. F., and Brasch, M. A. (2000). DNA cloning using in vitro site-specific recombination. Genome Research 10, 1788-1795. 

Corneille, S. et al. (2001). Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system. Plant. . 27 171-178. 

The chemistry of transposition is more complex than that of simple site-specific recombination. Transposition can occur at many sites in a genome, and no homology with the transposon termini is required. Transposition is accompanied by duplication of a short sequence of the recipient DNA exactly at the ends of the transposon. Usually 5, 9, or 11 base pairs are dupli-... 

Genetic modification of P. putida is highly relevant for metabolic engineering of producing strains. Modifications of interest consist of deletions and insertions attributing new features to the genome [66]. Such manipulations can be obtained by (i) homologous recombination (mediated by the RecA catalytic activity), (ii) site-specific recombination (subordinated to either the resolvase-invertase family or the FLP-fRT, Cre-loxP family, also referred to as the Int family), and... 

Herrmann, S., Siegl, T, Luzhetska, M., Petzke, L. et al. (2012) Site-specific recombination strategies for engineering actinomycete genomes. Appl Environ. Microbiol, 78 (6), 1804-1812. 

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