Real-time fluorescence

Redford, G. and Clegg, R. M. (2005). Real time fluorescence lifetime imaging and FRET using fast-gated image intensifiers. In Methods in... 

This problem is overcome by the Bio View sensor, which offers the possibility to monitor the whole spectral range simultaneously, and by using suitable data analysis and mathematical methods like chemometric regression models 11061. Real-time fluorescence measurement can be used more effectively comparing time-consuming off-line methods. Partial least squares (PLS) calibration models were developed for simultaneous on-line prediction of the cell dry mass concentration (Fig. 5), product concentration (Fig. 6), and metabolite concentrations (e. g., acetic acid, not shown) from 2D spectra. 

Aslanidis C, Schmitz G (1999) High-speed apolipoprotein E genotyping and apolipoprotein B3500 mutation detection using real-time fluorescence PCR and melting curves. Clin Chem 45 1094-1097... 

Sklar, L. A., Vilven, J., Lynam, E., Neldon, D., Bennett, T. A., and Prossnitz, E. (2000). Solubilization and display of G protein-coupled receptors on beads for real-time fluorescence and flow cytometric analysis. Biotechniques 28, 976-980, 982-985. Sklar, L. A., Edwards, B. S., Graves, S. W., Nolan, J. P., and Prossnitz, E. R. (2002). Flow cytometric analysis of ligand-receptor interactions and molecular assemblies. Annu. Rev. Biophys. Biomol. Struct. 31, 97—119. 

Mixing was characterized by an optical microscope with an epifluorescence attachment [160], A 10 4 M fluorescein solution in sodium phosphate buffer (10 mM, pH 7, with trace of methanol) was applied. A filter cube was used for fitting the excitation and emission characteristics, allowing selective passages of the radiation. A CCD digital camera collected real-time fluorescence images. 

In conclusion, the combination of localized and selective photoactivation of pa-GFP and 2PLSM allows the quantitative real-time fluorescence monitoring of dynamic intracellular processes in vivo that are of crucial importance for a deeper understanding... 

Figure 37-28 Finding the cycle number at which fluorescence rises above background. Real-time fluorescence data (F) from the amplification reaction are shown with the first (F ) and second (F ") derivatives.The maximum of the second derivative corresponds to a defined point on the real-time data where the curve starts to rise. (Modified with permission of the pubiisber from Wittwer CT, Kusukawa N. Real-time PCR. In Persing DH, Tenover FC,Versalovic J,TangYW, Unger ER, Reiman DA, White TJ (eds.), Molecular Microbiology Diagnostic Principles and Practice, Washington, DC ASM Press, 2004 71-84.)...

Lay MJ, Wittwer CT. Real-time fluorescence genotyp-mg of factor V Leiden during rapid-cycle PCR. Clin Chem 1997 43 2262-7. 

Palomares C, Torres Ml, Torres A, Aznar J, Palomares JC (2003) Rapid detection and identification of Staphylococcus aureus from blood culture specimens using real-time fluorescence PCR. Diagn Microbiol Infect Dis 45 183-189... 

Z. Ignatova, L. Gierasch, Monitoring protein stability and aggregation in vivo by real-time fluorescent labeling, Proc. Natl. Acad. Sri. 2004, 101, 523-528. 

Todd AV, et al. DzyNA-PCR use of DNAzymes to detect and quantify nucleic acid sequences in a real-time fluorescent format. Clin Chem 2000 46 625-630. 

Fig. 1. The real-time fluorescence intensity profiles of four representative samples tested for SNP marker rsl54162 during thermal cycling of the primer extension step of the TDl assay. R110 fluorescence is represented by solid circle and R6G fluorescence is represented by open circle. (A) shows the intensity profiles of a G/G homozygous sample (B) an A/A homozygous sample (C), a G/A heterozygous sample and (D), a negative control.

M7. Miyake, Y., Fujiwara, Y., Ohue, M., Yamamoto, H., Sugita, Y., Tomita, N., et al.. Quantification of micrometastases in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction. Int. J. Oncol 16, 289-293 (2000). 

FlexStation or FLIPR microplate reader with integrated pipetting system (Molecular devices), applied for ligand pipetting and real-time fluorescence measurement of calcium response. 

Lockey C, Otto E, Long Z (1998) Real-time fluorescence detection of a single DNA molecifle. BioTechniques 24 744-746... 

Figure 6. Sealed flow cell system for real-time fluorescence detection of PSi platform with enzymes attached.

Mizugaki M, Hiratsuka M, Agatsuma Y, Matsubara Y Fujii K, Kure S, Narisawa K (2000) Rapid detection of CYP2C18 genotypes by real-time fluorescence polymerase chain reaction. J Pharm Pharmacol 52 199-205... 

Figure 5.29 Real-time fluorescence assay for SPO (left) and PGM (right) showing the different selectivity of the BBV/HPTS probes. The enzymatic reactions are both followed with 3,3 -o-BBV (o) and 4,4 -o-BBV ( ). Background with no enzyme (x) using either 4,4 -o-BBV (SPO, left) or 3,3 -o-BBV (PGM, right) as the receptor.

K. Kiyose, K. Hanaoka, D. Oushiki, T. Nakamura, M. Kajimura, M. Suematsu, H. Nishimatsu, T. Yamane, T. Terai, Y. Hirata, and T. Nagano, Hypoxia-sensitive fluorescent probes for in vivo real-time fluorescence imaging of acute ischemia, / Am Chem Soc, 132 (45), 15846-8,2010. 

K. -G. Wahlund, K, Mosbach, A. Miyabayashi, P.-O. Larsson Real-time fluorescence imaging of capillary electrophoresis Separation of nucleic acids, J. Cap. Elec. 22, 46 (1995)... 

The cell distribution of 3-amino-4-hydroxymethyl acridine derivatives 70 (Scheme 25), which has the N3—C4—16 substitution pattern, was studied by real-time fluorescence microscopy and SIMS structured illumination microscopy). The latter method required the introduction of an iodine atom at position 6 of the acridine which influences the Upophihcity but does not modify significantly the biological properties such as IC50 and subcellular localization (2009EJMC4758). A co-polymer 71 consisting of water-soluble maleic anhydride-containing poly[maleic anhydride-u/i-acrylic acid] (poly(MA-alt-AA) or MAAA) copolymer was modified with acriflavine (AF) which displayed antibacterial activity on EHEC and Staphylococcus aureus (2014MI2903). 

Matlock, D. L. Heyduk, T. A real-time fluorescence method to monitor the melting of duplex DNA during... 

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