Reagent layer

Reagent layer 1 porous gelatin coating film of urease and pH buffer... 

Reagent layer 2 porous cellulose acetate coating film having a pH indicator... 

Upper ihde mount Spreading layer Reagent layer Sew i per we able lay e r Indicator layer Support... 

An essential component of a molecular sensor consists of reagent layers. Creation of these layers requires the immobilization of recognition elements for the detection method (Sharma et al., 2003). 

The effect of increasing temperature is to increase mass transport rates for all categories of diffusion. The obvious implication of more rapid mass transport for equilibrium-based interactions is more rapid sensor response. In addition, sensors based on the consumption of a reagent layer generally show enhanced sensitivity with increased temperature, because reaction rates and diffusion rates both exhibit a positive Arrhenius temperature dependence. 

Carrier layer Reagent layer Spreading layer... 

The reagent layer comprises all the components required for completing the reaction. It is uniformly coated on the support base, to which it is affixed by a water-soluble polymeric binder. The sample extends uniformly over the reagents, thus enabling the reaction to take place. The chemicals within the reagent layer vary with the analyte being measured. The support base material is a polyester film, of the same type as the films used for photography, with a smooth and uniform surface. 

Reflectance values on Cobas Ready as on any diffuse reflector are measured as the quantities of light reflected from a reagent layer saturated with a serum sample (/,) or from a white or black reference plate (/ and 4), each normalized to the incident light intensity monitored by the reference photodetector... 

Spreading layer Reflectance layer Reagent layer Porous filter Indicator layer Carrier layer... 

Storage, changes taking place in the gelatine matrix (reagent layer) will render the slides unusable. 

After the dye has formed in the reagent layer the slide can be assessed reflectometrically. Since the construction of the reflectometer is not the same with all Kodak instruments, the following is a description in principal only. For details of the technique of measurement see the sections describing the individual instruments. 

Reagent layer Potossium ferricyanide K Phosphote buffer. pH 76 ... 

Reagent layer Buffer, pH 7.0 ADP, glycerol, peroxidase Magnesium acetate Diadenosine pentaphosphate Glycerol kinase Leuco dye Glycerophosphate oxidase... 

Reagent layer Buffer. pH 70, creatine phosphate, ADP, magnesium acetate, diadenosine penta-phosphate, glycerol, peroxidase, glycerol kinase, leuco dye precursor, L-a-glycero-phosphate oxidase, AMP, creatine phosphate ... 

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