Assay, radioligand

Christopoulos, A. (2000). Quantification of allosteric interactions at G-protein coupled receptors using radioligand assays. In Current protocol in pharmacology, edited by Enna, S. J., pp. 1.22.21-1.22.40. Wiley and Sons, New York. 

Flow cytometric evaluation of fluorescent chemoattractant binding to leukocytes offers several advantages over conventional radioligand assays ... 

Daughaday WH, Kapadia M, Mariz I Serum somatomedin binding proteins physiologic significance and interference in radioligand assay./. Lab. Clin. Med. (1987) 109 355-363. 

Rodbard D, Frazier GR Statistical analysis of radioligand assay data. Methods Enzy-mol. (1975) 37 3-22. 

Seissler J, Boms S, Wohlrab U, Morgenthaler NG, Mothes T, Boehm BO, et al. Antibodies to human recombinant tissue transglutaminase measured by radioligand assay Evidence for high diagnostic sensitivity for celiac disease. Horm Metab Res 1999 31 375-379. 

DeLean A, Munson PJ, Rodbard D (1978) Simultaneous analysis of families of sigmoidal curves Application to bioassay, radioligand assay, and physiological dose-response curves. Am J Physiol 235 E97-E102... 

Ratcliffe WA, Corrie JE, Dalziel AH, Macpherson JS. Direct T-radioligand assays for serum progesterone compared with assays involving extraction of serum. Clin Chem 1982 28 1314-18. 

Christopoulos, A. (2000). Quantification of allosteric interactions at G-protein coupled receptors using radioligand assays. In Current... 

In laboratories which use an NSILA bioassay, however, somatomedin activity has been expressed in units of insulinlike activity, generally p,U/ml, a convention which has to some extent continued as radioligand assays were introduced. Expressed in these units, normal human samples have a mean value of 350 66 pU/ml by competitive protein-binding assay (Z4). In the same laboratory, a mean normal value ( SD) of 148 45 ng/ml was determined by IGF-I RIA (Z5). The close correspondence between reference values obtained by RIA in different laboratories, when expressed as ng/ml of SM-C or IGF-I, suggests that mass units, if uniformly adopted, would allow better comparison of results. With the increasing availability of highly pu-... 

Gluckman, P. D., Johnson-Barrett, J. J., Butler, J. H., Edgar, B. W.. and Gunn, T. R., Studies of insulin-like growth foctor-I and -H by specific radioligand assays in umbilical cord blood. CUn. Endocrinol. (Chford) 19, 405-413 (1983). 

V3. Van Wyk, ]. J., Svobo M. E., and Underwood, L. E., Evidence from radioligand assays that somatomedin-C and insulin-like growth fiictor-1 are similar to each other and diC ent from other somatomedins. /. CEn. Endocrinol. Metab. 50, 206-206 (1980). 

D Rodbard, JE Lewald. In E Diczfalusy, A Diczfalusy, eds. Computer analysis of radioligand assay and radioimmunoassay data. Steroid Assay by Protein Binding. Karolinska Symposia on Research Methods in Reproductive Endocrinology, WHO/ Karolinska Institut, Stockholm, 1970, p 79. 

D Rodbard, PJ Munson, A De Lean. Improved curve-fitting, parallelism testing, characterisation of sensitivity and specificity, validation, and optimization for radioligand assays. In Radioimmunoassay and Related Procedures in Medicine 1, Vienna International Atomic Energy Agency, 1978, p 469. 

D Rodbard, JE Lewald. Computer analysis of radioligand assay and radioimmunoassay data. Acta Endocrinol suppl 64(147) 79, 1970. 

Vitamin D and its metabolites can be detected on the basis of their native UV absorption (Amax = 264 nm) or after conversion to isotachysterols (Amax = 301 nm). However, because of the inherent poor selectivity and sensitivity of this approach quantitation is mostly performed offline by a radioligand assay. Several metabolites have also been determined in plasma by LC-thermospray MS. 

Radioligand assays 25-OHD3 Plasma Deproteinization acetonitrile Nucleosil-10-NO2 10 pm Gradient-hexane -> hexane- CPB... 

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