Radioligand binding assays, problems

It had been shown that 50 mM Tris-citrate buffer with 1 M NaCl was a suitable incubation buffer for the binding to GATl [95], The high salt concentration in this incubation buffer caused no problems in the new MS binding assays, since most of the incubation buffer was removed before the liberation step. The separation of the bound from the nonbound marker was conducted by filtration over glass fiber filters as common in radioligand binding assays. 

Measurement of the serum or red blood cell concentration of folate is the method of choice, and a number of simple and reliable radioligand-binding assays have been developed. There are a number of problems involved in radioligand-binding assays for folate, and in some centres microbiological determination of plasma or whole-blood folates is the preferred technique. 

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